3,3'-Diaminobenzidine is used as peroxidase substrate and a reagent for spectrophotometric determination of selenium. DAB is used for the immunohistochemical staining of nucleic acids and proteins. As a dark brown dye, was used as an antibody-specific stain to identify the paired antibodies in breast tissue. It may be used for the synthesis of a useful, linear, Schiff-base coordination polymer.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Applications
3,3′-Diaminobenzidine is used as peroxidase substrate and a reagent for spectrophotometric determination of selenium. DAB is used for the immunohistochemical staining of nucleic acids and proteins. As a dark brown dye, was used as an antibody-specific stain to identify the paired antibodies in breast tissue. It may be used for the synthesis of a useful, linear, Schiff-base coordination polymer.
Solubility
Soluble in water (partly).
Notes
Light sensitive. Incompatible with oxidizing agents and heat.
RUO – Research Use Only
General References:
- N.H.Chua. Photooxidation of 3,3'-diaminobenzidine by blue-green algae and Chlamydomonas reinhardii. Biochimica et Biophysica Acta. 1972, 267, (1),179-189
- Volker Herzog; H.Dariush Fahimi. A new sensitive colorimetric assay for peroxidase using 3,3'-diaminobenzidine as hydrogen donor. Analytical Biochemistry. 1973, 55, (2),554-562