Image-iT™ LIVE Red and Green Caspase Apoptosis Detection Kits for microscopy
Image-iT™ LIVE Red and Green Caspase Apoptosis Detection Kits for microscopy
Actual product may vary
Invitrogen™

Image-iT™ LIVE Red and Green Caspase Apoptosis Detection Kits for microscopy

Detect apoptosis via microscopy, including fluorescence microscopy and HCS, with Image-iT LIVE Red and Green Caspase Apoptosis Detection Kits that report poly and caspase-3/7 activities.
Have Questions?
Change viewbuttonViewtableView
Catalog NumberColorEnzymeLabel or Dye
I35101Red, Green, BluePoly CaspasesSR-VAD-FMK, SYTOX Green, Hoechst 33342
I35102Red, Green, BlueCaspase 3/7SR-DEVD-FMK, SYTOX Green, Hoechst 33342
I35104Green, Red, BluePoly CaspasesFAM-VAD-FMK, Propidium Iodide, Hoechst 33342
I35106Green, Red, BlueCaspase 3/7FAM-DEVD-FMK, Propidium Iodide, Hoechst 33342
Catalog number I35101
Price (USD)
512.00
Each
Add to cart
Color:
Red, Green, Blue
Enzyme:
Poly Caspases
Label or Dye:
SR-VAD-FMK, SYTOX Green, Hoechst 33342
Price (USD)
512.00
Each
Add to cart
Ask our AI about this Product
Detect and evaluate caspase activation, membrane permeability, and cell cycle using Image-iT LIVE red and green poly and caspase-3 and-7 apoptosis detection kits for microscopy, including fluorescence microscopy and high content screening (HCS). The Image-iT LIVE red and green caspase detection kits use fluorescent inhibitor of caspases (FLICA) methodology to detect and report caspase activity, a measure of cell apoptosis. Specifically, the Image-iT LIVE red and green caspase detection kits contain the substrates SR-VAD-FMK and SR-DEVD-FMK (for red fluorescence), or FAM-VAD-FMK and FAM-DEVD-FMK (green fluorescence), for poly caspases and caspase-3/7 detection, respectively. They also include Hoechst 33342 and propidium iodide stains, enabling simultaneous evaluation of membrane permeability and cell cycle by microscopy and HCS.
The Image-iT LIVE red and green caspase detection kits for microscopy employ a novel approach to detecting active caspases: both assay kits take advantage of a fluorescent inhibitor of caspases (FLICA) methodology, which is an affinity label. This reagent associates a fluoromethyl ketone (FMK) moiety, which can react covalently with a cysteine, with a caspase-specific amino acid sequence. Different amino acid recognition sequences are used to detect different caspases: valine-alanine-aspartic acid (VAD) for poly-caspases (including caspase-1, -3, -4, -5, -6, -7, -8, and -9), and aspartic acid-glutamic acid-valine-aspartic acid (DEVD) for caspase 3/7. A sulforhodamine (SR) group or carboxyfluorescein (FAM) group is attached as a reporter.

The FLICA reagent is thought to interact with the enzymatic reactive center of an activated caspase via the recognition sequence, and then to attach covalently through the FMK moiety. The FLICA inhibitor is cell permeant and noncytotoxic. Unbound FLICA molecules diffuse out of the cell and are washed away; the remaining red or green fluorescent signal is a direct measure of the amount of active caspase present at the time the inhibitor was added. The approximate excitation and emission peaks of the FLICA, propidium iodide, and Hoechst 33342 reagents are 488⁄ nm/530 nm, 535⁄ nm/6617 nm, and 350 nm/⁄461 nm, respectively. The detection reagents included within the Image-iT LIVE Red Poly Caspases, Red Caspase-3/7, Green Poly Caspases, and Green Caspase-3/7 Detection kits are SR-VAD-FMK, SR-DEVD-FMK, FAM-VAD-FMK, and FAM-DEVD-FMK, respectively.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorRed, Green, Blue
DescriptionImage-iT LIVE Red Poly Caspases Detection Kit
EnzymePoly Caspases
Excitation/EmissionSR-VAD-FMK: 550/595
SYTOX Green: 504/523
Hoechst 33342: 350/461
For Use With (Equipment)Fluorescence Microscope
Label TypeOther Label(s) or Dye(s)
Label or DyeSR-VAD-FMK, SYTOX Green, Hoechst 33342
No. of Reactions25 tests (labeling volumes of 300μL)
Product LineImage-iT
Product TypeCaspase Assay Kit
Quantity1 kit
Shipping ConditionRoom Temperature
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
Detection MethodFluorescence
FormatSlide
Unit SizeEach
Have questions about this product? Ask our AI assisted search.
This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our Privacy Notice.

Documents & Downloads

Certificates

Safety Data Sheets

Share catalog number, name or link