iBlot™ 2 Transfer Stacks, PVDF, regular size
Limited time savings on remaining stock of iBlot 2 devices! While supplies last. Save now!
Like the iBlot 2 Dry Blotting System? Then you'll love our iBlot 3 Dry Blotting System which enables twice the throughput and features optional built-in cooling for better consistency. Learn more about the benefits of the iBlot 3 here.
Share
iBlot™ 2 Transfer Stacks, PVDF, regular size
Invitrogen™

iBlot™ 2 Transfer Stacks, PVDF, regular size

Green features
iBlot 2 Transfer Stacks are used to transfer proteins using the iBlot 2 Gel Transfer Device. iBlot 2 Transfer StacksRead more
Have Questions?
Catalog NumberQuantity
IB2400110 Stacks
Catalog number IB24001
Price (USD)
305.65
Online Exclusive
329.00
Save 23.35 (7%)
Each
Add to cart
Quantity:
10 Stacks
Price (USD)
305.65
Online Exclusive
329.00
Save 23.35 (7%)
Each
Add to cart
Ask our AI about this Product

iBlot 2 Transfer Stacks are used to transfer proteins using the iBlot 2 Gel Transfer Device. iBlot 2 Transfer Stacks are consumable stacks with an integrated pre-activated PVDF transfer membrane for dry blotting of proteins. Each iBlot 2 Transfer Stack contains a copper-coated electrode and appropriate cathode and anode buffers in the gel matrix to allow fast, reliable transfer of proteins. This regular-size stack (13 x 8.3 cm) can be used with midi-size gels or two mini-size gels or E-PAGE 48 or 96 gels.

PVDF membrane

The PVDF membrane (0.2-μm pore size, low fluorescence) in this stack has higher binding capacity than nitrocellulose. It is pre-activated and ready for use without any pretreatment with alcohols. The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, and radiolabeling. The proteins bind to the membrane due to hydrophobic interactions. The protein-binding capacity is 240 μg/cm2.

WARNING: Reproductive Harm - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Green FeaturesLess waste, responsibly packaged, extended life
Quantity10 Stacks
Dimensions (LxW)13.5 x 8 cm
FormatTransfer Stack
Length (Metric)13.5 cm
MaterialPVDF
Pore Size0.2 μm
Product LineiBlot
Sufficient For10 Blots
Width (Metric)8 cm
Unit SizeEach
Contents & Storage
Store at room temperature.
iBlot 3 Western Blot Transfer System

Introducing iBlot 3 Western Blot Transfer System

Featuring higher throughput and built-in cooling for consistent protein transfer
Learn more ›

Have questions about this product? Ask our AI assisted search.
This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our Privacy Notice.

Frequently asked questions (FAQs)

If a green color appears on the membrane after the transfer using the iBlot 2 Dry Blotting System, will it affect the results?

The green discoloration is copper deposits from the transfer stack, and it does not affect the results. To minimize this effect, shake excess water off the filter paper and buffer from the gel before placing each on the stack. The current formulation of stacks minimizes the green discoloration.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I cut the bottom stack of my iBlot 2 Transfer Stack to fit my separating gel?

No. Do not trim the iBlot 2 Transfer Stacks to fit your gel size.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Is the PVDF membrane in the iBlot 2 PVDF Gel Transfer Stack pre-activated?

The PVDF membrane is pre-activated and ready for use without any pre-treatment with alcohol.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the stack and/or absorbent pad more than once when using the iBlot 2 Dry Blotting System?

No. The transfer stacks have a finite amount of ions to drive the transfer and are depleted after a single use.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I perform multiple transfers, with a new transfer starting immediately after the previous one when using the iBlot 2 Dry Blotting System?

Yes. The device is designed to do so with no impact on performance.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all iBlot™ 2 Transfer Stacks, PVDF, regular size FAQs

Documents & Downloads

Certificates

Search by lot number or partial lot number
Search
Request a Certificate

Safety Data Sheets

Share catalog number, name or link