Anza™ 8 XhoI
Anza™ 8 XhoI
Invitrogen™

Anza™ 8 XhoI

5' C ↓ T C G A G 3'3' G A G C T ↑ C 5'Invitrogen™ Anza™ 8 XhoIRead more
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Catalog NumberQuantity
IVGN00864000 units
IVGN008820,000 units
Catalog number IVGN0086
Price (USD)
161.00
Each
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Quantity:
4000 units
Price (USD)
161.00
Each
Add to cart
5'   C ↓ T    C    G    A    G   3'
3'   G    A    G    C    T ↑ C   5'


Invitrogen™ Anza™ 8 XhoI is a restriction enzyme that cuts DNA at this recognition site: C^TCGAG, completely digesting the DNA in 15 minutes at 37°C. Isoschizomers include: PaeR7I, Sfr274I, SlaI.

For superior convenience, a single buffer and protocol are used with all Anza™ restriction enzymes. The single Anza buffer allows multiple restriction enzymes to be used simultaneously in one reaction. This helps eliminate the need for sequential digests and the DNA clean-up necessary when changing buffers. Additionally, the Anza buffer and restriction enzymes have been designed for flexibility in digestion times. Digestions of up to 16 hours can be carried out without star activity.

Convenient—all Anza enzymes are 100% functional in the single Anza buffer
Fast—complete digestion in 15 minutes
Flexible—overnight digestion without star activity
Simple—a single digestion protocol for all DNA types
Complete system—incorporates Anza DNA Modifying Enzymes for a complete cloning experience

Anza DNA Modifying Enzymes include the Anza T4 DNA Ligase Master Mix, Anza Alkaline Phosphatase Kit, Anza T4 PNK Kit, Anza DNA Blunt End Kit, and Anza DNA End Repair Kit. Use these enzymes for a complete cloning workflow.

Anza Red Buffer, which contains a density gradient and tracking dyes, is supplied for added convenience. Digestion reactions are as efficient in the Anza Red Buffer as in the Anza Buffer. Use of the Anza Red Buffer helps reduce pipetting steps when preparing multiple digestion reactions and a subsequent step includes agarose gel electrophoresis. For applications that require product analysis by fluorescence excitation, the colorless Anza Buffer without the tracking dyes is recommended.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Compatible BufferAnza™ 10X Red Buffer, Anza™ 10X Buffer
Product TypeRestriction Enzyme Cloning System
Quantity4000 units
Shipping ConditionApproved for shipment on Wet or Dry Ice
EnzymeXho I
Methylation SensitivityCpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Optimal Reaction Temperature37°C
Product LineAnza™
Research CategoryTraditional Cloning
Sensitive to Heat InactivationYes
Type IIS RENo
Unit SizeEach
Contents & Storage
200 μL Anza 8 XhoI
500 μL Anza Buffer (10X)
500 μL Anza Red Buffer (10X)

Store at -20°C.

Frequently asked questions (FAQs)

Do Anza restriction enzymes contain BSA?

All Anza restriction enzymes contain BSA.

Are Anza enzymes stable during shipment if they freeze and thaw?

Enzymes may freeze during shipment on dry ice. This does not affect them as they have been tested and shown to be 100% active after three freeze-thaw cycles. For 24-48 hour delivery, enzymes may be shipped on ice pack as their performance is not affected by short exposure times at 4 degrees C.

Is it possible to perform a double digestion using an Anza restriction enzyme and a FastDigest restriction enzyme or Thermo Scientific restriction enzyme that is not available in the Anza collection?

For optimal results, we highly recommend exclusively using Anza restriction enzymes in double digestion as they all have 100% buffer compatibility. If a certain enzyme is not available in Anza format, but is available in FastDigest format or as a Thermo Scientific conventional restriction enzyme, it may be possible to perform a double digestion. For specific protocol recommendations, please contact our technical support with detailed information of the desired enzymes and DNA template.

Why does the Anza restriction enzyme come with a clear buffer and a red buffer?

When the next step following digestion is agarose gel electrophoresis, the Anza Red buffer can be used directly. The Anza Red buffer contains the necessary loading dye and density gradient for directly loading on to the agarose gel. The red dye does not interfere with digestion and helps reduce additional pipetting.

Use the clear Anza Buffer for applications that require analysis by fluorescence excitation, as the dyes in the Anza Red Buffer may interfere with some fluorescence measurements.

Why is there a number in the name of the Anza restriction enzymes?

Anza restriction enzymes contain a number preceding the name. This number provides the option to organize the enzymes in freezers numerically or alphabetically. More common enzymes tend to have a lower number.

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