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Anza™ 73 BclI
Anza™ 73 BclI
Invitrogen™

Anza™ 73 BclI

5' T ↓ G A T C A 3'3' A C T A G ↑ T 5'Invitrogen™ Anza™ 73 BclIRead more
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Catalog NumberQuantity
IVGN07362500 units
Catalog number IVGN0736
Price (USD)
153.00
Each
Estimated availability date 28-Jul-2025
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Quantity:
2500 units
Recurring order eligible. Learn more »
Price (USD)
153.00
Each
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5'    T ↓ G    A    T    C    A    3'
3'    A    C    T    A    G ↑ T    5'


Invitrogen™ Anza™ 73 BclI is a restriction enzyme that cuts DNA at this recognition site: T^GATCA, completely digesting the DNA in 15 minutes at 37°C. Isoschizomers include: FbaI, Ksp22I.

For superior convenience, a single buffer and protocol are used with all Anza™ restriction enzymes. The single Anza buffer allows multiple restriction enzymes to be used simultaneously in one reaction. This helps eliminate the need for sequential digests and the DNA clean-up necessary when changing buffers. Additionally, the Anza buffer and restriction enzymes have been designed for flexibility in digestion times. Digestions of up to 16 hours can be carried out without star activity.

Convenient—all Anza enzymes are 100% functional in the single Anza buffer
Fast—complete digestion in 15 minutes
Flexible—overnight digestion without star activity
Simple—a single digestion protocol for all DNA types
Complete system—incorporates Anza DNA Modifying Enzymes for a complete cloning experience

Anza DNA Modifying Enzymes include the Anza T4 DNA Ligase Master Mix, Anza Alkaline Phosphatase Kit, Anza T4 PNK Kit, Anza DNA Blunt End Kit, and Anza DNA End Repair Kit. Use these enzymes for a complete cloning workflow.

Anza Red Buffer, which contains a density gradient and tracking dyes, is supplied for added convenience. Digestion reactions are as efficient in the Anza Red Buffer as in the Anza Buffer. Use of the Anza Red Buffer helps reduce pipetting steps when preparing multiple digestion reactions and a subsequent step includes agarose gel electrophoresis. For applications that require product analysis by fluorescence excitation, the colorless Anza Buffer without the tracking dyes is recommended.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Compatible BufferAnza™ 10X Red Buffer, Anza™ 10X Buffer
Product TypeRestriction Enzyme Cloning System
Quantity2500 units
Shipping ConditionApproved for shipment on Wet or Dry Ice
EnzymeBclI
Methylation SensitivityDam Methylation-Sensitive, Not CpG Methylation-Sensitive, Not Dcm Methylation-Sensitive
Optimal Reaction Temperature37°C
Product LineAnza™
Research CategoryTraditional Cloning
Sensitive to Heat InactivationYes
Type IIS RENo
Unit SizeEach
Contents & Storage
250 μL Anza 73 BclI
500 μL Anza Buffer (10X)
500 μL Anza Red Buffer (10X)

Store at -20°C.
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Documents & Downloads

Certificates

Lot #Certificate TypeDateCatalog Number(s)
3237869Certificate of AnalysisMay 13, 2025IVGN0736
3171417Certificate of AnalysisFeb 14, 2025IVGN0736
3103748Certificate of AnalysisJan 31, 2025IVGN0736
2835739Certificate of AnalysisJan 29, 2025IVGN0736
2867602Certificate of AnalysisJan 28, 2025IVGN0736
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Safety Data Sheets

Frequently asked questions (FAQs)

All Anza restriction enzymes contain BSA.

Enzymes may freeze during shipment on dry ice. This does not affect them as they have been tested and shown to be 100% active after three freeze-thaw cycles. For 24-48 hour delivery, enzymes may be shipped on ice pack as their performance is not affected by short exposure times at 4 degrees C.

For optimal results, we highly recommend exclusively using Anza restriction enzymes in double digestion as they all have 100% buffer compatibility. If a certain enzyme is not available in Anza format, but is available in FastDigest format or as a Thermo Scientific conventional restriction enzyme, it may be possible to perform a double digestion. For specific protocol recommendations, please contact our technical support with detailed information of the desired enzymes and DNA template.

When the next step following digestion is agarose gel electrophoresis, the Anza Red buffer can be used directly. The Anza Red buffer contains the necessary loading dye and density gradient for directly loading on to the agarose gel. The red dye does not interfere with digestion and helps reduce additional pipetting.

Use the clear Anza Buffer for applications that require analysis by fluorescence excitation, as the dyes in the Anza Red Buffer may interfere with some fluorescence measurements.

Anza restriction enzymes contain a number preceding the name. This number provides the option to organize the enzymes in freezers numerically or alphabetically. More common enzymes tend to have a lower number.

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