3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate is a chromophore substrate in ELISA assays. It is a noncarcinogenic substitute for benzidine.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Applications
3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate is a chromophore substrate in ELISA assays. It is a noncarcinogenic substitute for benzidine.
Solubility
Soluble in DMSO (∼1.7 mg/ml), and 1:300 DMSO:PBS (pH 7.2) (0.03 mg/ml).
Notes
Moisture and light sensitive. Store away from oxidizing agents, heat, light, acids, water/ moisture.
RUO – Research Use Only
General References:
- Sarah Gerlo; Peggy Verdood; Birgit Gellersen; Elisabeth L. Hooghe-Peters and Ron Kooijman. Mechanism of Prostaglandin (PG)E2-Induced Prolactin Expression in Human T Cells: Cooperation of Two PGE2 Receptor Subtypes, E-Prostanoid (EP) 3 and EP4, Via Calcium- and Cyclic Adenosine 5'-Monophosphate-Mediated Signaling Pathways. The Journal of Immunology.2004, 173 (10), 5952-5962.
- Evemie Schutyser; Sofie Struyf; Patricia Menten; Jean-Pierre Lenaerts; René Conings; Willy Put; Anja Wuyts; Paul Proost and Jo Van Damme. Regulated Production and Molecular Diversity of Human Liver and Activation-Regulated Chemokine/Macrophage Inflammatory Protein-3α from Normal and Transformed Cells. The Journal of Immunology. 1973, 165 (8), 4470-4477.