o-Phenylenediamine dihydrochloride, 98+%, Thermo Scientific Chemicals
o-Phenylenediamine dihydrochloride, 98+%, Thermo Scientific Chemicals
Thermo Scientific Chemicals

o-Phenylenediamine dihydrochloride, 98+%, Thermo Scientific Chemicals

A peroxidase substrate for ELISA procedures
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Quantity:
100 g
25 g
Catalog number J60354.22
also known as J60354-22
Price (USD)/ Each
277.00
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Quantity:
100 g
Request bulk or custom format
Price (USD)/ Each
277.00
Add to cart
o-Phenylenediamine dihydrochloride, 98+%, Thermo Scientific Chemicals
Catalog numberJ60354.22
Price (USD)/ Each
277.00
-
Add to cart
Chemical Identifiers
CAS615-28-1
IUPAC Namedihydrogen benzene-1,2-diamine dichloride
Molecular FormulaC6H10Cl2N2
InChI KeyRIIWUGSYXOBDMC-UHFFFAOYSA-N
SMILES[H+].[H+].[Cl-].[Cl-].NC1=CC=CC=C1N
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SpecificationsSpecification SheetSpecification Sheet
Assay (HPLC)>98.0%
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.

Solubility
Slightly soluble in water; freely soluble in alcohol, chloroform or ether

Notes
Store in cool, dry place in a tightly sealed container. Store away from oxidizing agents, heat. Store in refrigerator.
WARNING: Cancer – www.P65Warnings.ca.gov
RUO – Research Use Only

General References:

  1. J H Bovaird; T T Ngo; H M Lenhoff. Optimizing the o-phenylenediamine assay for horseradish peroxidase: effects of phosphate and pH, substrate and enzyme concentrations, and stopping reagents. Clinical Chemistry.1982, 28 (12), 2423-2426.
  2. G Wolters; L Kuijpers; J Kacaki; A Schuurs.Solid-phase enzyme-immunoassay for detection of hepatitis B surface antigen. Journal of Clinical Pathology. 1976, 29 (10), 873-879.