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HEPES-buffered saline, pH 7.0 (5X)
HEPES-buffered saline, pH 7.0 (5X)
HEPES-buffered saline, pH 7.0 (5X)
HEPES-buffered saline, pH 7.0 (5X)
Thermo Scientific Chemicals

HEPES-buffered saline, pH 7.0 (5X)

CAS: 7365-45-9 | C8H18N2O4S | 238.30 g/mol
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250 mL
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Catalog number J60753.AP
also known as J60753-AP
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500 mL
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Chemical Identifiers
CAS7365-45-9
IUPAC Name2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid
Molecular FormulaC8H18N2O4S
InChI KeyJKMHFZQWWAIEOD-UHFFFAOYSA-N
SMILESOCCN1CCN(CCS(O)(=O)=O)CC1
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SpecificationsSpecification SheetSpecification Sheet
FormClear, colorless liquid
pH7.0 ± 0.15
MethodUltrapure ingredients in given concentration was dissolved in 18.2 megohn-cm ± 1 water (CDl, 18 megohm, UV treated), adjusted to pH 7.0, and then filtered through 0.22 micron filter.
HEPES is widely used in cell culture. HEPES is used to neutralize the complex proteins in growth medium that may inactivate trypsin during trypsinization. N-2-hydroxyethylpiperazine-N-2?-ethanesulfonic acid (Hepes) has been tested as an alternative to tris-(hydroxymethyl)aminoethane (Tris) for hydrogen-ion buffering of algal cultures. Separation of human platelets from plasma proteins including factor VIII VWF by a combined albumin gradient-gel filtration method by using HEPES buffer. May be used in isoelectric focusing applications and as a buffer in the quantitative and selective measurement of antigen-antibody reactions.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
HEPES is widely used in cell culture. HEPES is used to neutralize the complex proteins in growth medium that may inactivate trypsin during trypsinization. N-2-hydroxyethylpiperazine-N-2′-ethanesulfonic acid (Hepes) has been tested as an alternative to tris-(hydroxymethyl)aminoethane (Tris) for hydrogen-ion buffering of algal cultures. Separation of human platelets from plasma proteins including factor VIII VWF by a combined albumin gradient-gel filtration method by using HEPES buffer. May be used in isoelectric focusing applications and as a buffer in the quantitative and selective measurement of antigen-antibody reactions.

Solubility
Soluble in H2O: 1 M at 20°C, clear, colorless

Notes
Contains 125mM HEPES and 750mM sodium chloride, at pH 7.0
RUO – Research Use Only

General References:

  1. Charles Shipman Jr. Evaluation of 4-(2-Hydroxyethyl)-1-piperazineëthanesulfonic Acid (HEPES) as a Tissue Culture Buffe. Exp Biol Med (Maywood) January 1969. 1969, 130 (1), 305-310.
  2. G. I. McFadden,; M. Melkonian. Use of Hepes buffer for microalgal culture media and fixation for electron microscopy. Journal name. 1986, 25 (4), 551-557..

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