n-Octyl-beta-D-thioglucopyranoside is used for cell lysis or to solubilise membrane proteins without denaturing them. This is particularly of use in order to crystallise them or to reconstitute them into lipid bilayers. It has been used in a study to assess the crystallization and preliminary x-ray analysis of human plasma apolipoprotein C-I. It has also been used in a study that investigated the identification of Chlamydia pneumoniae proteins.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Applications
n-Octyl-beta-D-thioglucopyranoside is used for cell lysis or to solubilise membrane proteins without denaturing them. This is particularly of use in order to crystallise them or to reconstitute them into lipid bilayers. It has been used in a study to assess the crystallization and preliminary x-ray analysis of human plasma apolipoprotein C-I. It has also been used in a study that investigated the identification of Chlamydia pneumoniae proteins.
Solubility
Soluble in ethanol, water or methanol
Notes
Stable under recommended storage conditions. Incompatible with oxidizing agents.
RUO – Research Use Only
General References:
- K.H.Weisgraber; Y.M.Newhouse; A.McPherson. Crystallization and preliminary X-ray analysis of human plasma apolipoprotein C-I. Journal of Molecular Biology. 1994, 236,(1), 382-384.
- Sanghamitra Mukhopadhyay; David Good; Richard D.Miller; James E.Graham; Sarah A.Mathews; Peter Timms; James T.Summersgill. Identification of Chlamydia pneumoniae proteins in the transition from reticulate to elementary body formation. Molecular & Cellular Proteomics. 2006, 5,(12), 2311-2318.