3,3',5,5'-Tetramethylbenzidine soln., Ready-to-Use, high sensitivity, Thermo Scientific Chemicals
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3,3',5,5'-Tetramethylbenzidine soln., Ready-to-Use, high sensitivity, Thermo Scientific Chemicals
Thermo Scientific Chemicals

3,3',5,5'-Tetramethylbenzidine soln., Ready-to-Use, high sensitivity, Thermo Scientific Chemicals

3,3’,5,5’-Tetramethylbenzidine (TMB, CAS # 54827-17-7) is a blue chromogenic substance used in staining techniques such as immunohistochemistry.
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Catalog number J61325.AU
also known as J61325-AU
Price (USD)/ Each
412.65
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Ends: 31-Dec-2024
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Quantity:
1000mL
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Price (USD)/ Each
412.65
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Online exclusive
Ends: 31-Dec-2024
485.00 
Save 72.35 (15%)
Add to cart
3,3',5,5'-Tetramethylbenzidine soln., Ready-to-Use, high sensitivity, Thermo Scientific Chemicals
Catalog numberJ61325.AU
Price (USD)/ Each
412.65
Special Offer
Online exclusive
Ends: 31-Dec-2024
485.00 
Save 72.35 (15%)
-
Add to cart
Chemical Identifiers
CAS54827-17-7
IUPAC Name3,3',5,5'-tetramethyl-[1,1'-biphenyl]-4,4'-diamine
Molecular FormulaC16H20N2
InChI KeyUAIUNKRWKOVEES-UHFFFAOYSA-N
SMILESCC1=CC(=CC(C)=C1N)C1=CC(C)=C(N)C(C)=C1
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SpecificationsSpecification SheetSpecification Sheet
Appearance (Color)Clear colorless to pale blue
pH6.0 ± 0.2
Assay (special)Microwell test: ± 6% of control
ClarityTransparent
FormLiquid
TMB is a substrate for horseradish peroxidase. Recommended for ELISA (microwell) procedures,3,3?,5,5?-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3?,5,5?-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

• TMB is an aromatic amine that acts as a hydrogen donor in horseradish peroxidase-catalyzed reactions
• The transfer of hydrogen results in a blue stain associated with the target of interest
• This pH-dependent color change can be confirmed using spectrophotometric methods
• TMB is commonly used in immunohistochemistry; TMB is light-sensitive, and all applications should be performed in the dark

Application

• TMB is suitable for immunohistochemistry and immunocytochemistry applications including ELISA and cell and tissue staining

WARNING: Reproductive Harm - www.P65Warnings.ca.gov
RUO – Research Use Only

Literature References

Zhang X, Yang Q, Lang Y, Jiang X, Wu P. Rationale of 3,3',5,5'-Tetramethylbenzidine as the Chromogenic Substrate in Colorimetric Analysis. Anal Chem. 2020 Sep 15;92(18):12400-12406. doi: 10.1021/acs.analchem.0c02149. Epub 2020 Aug 31. PMID: 32794705.

Palladino P, Torrini F, Scarano S, Minunni M. 3,3',5,5'-tetramethylbenzidine as multi-colorimetric indicator of chlorine in water in line with health guideline values. Anal Bioanal Chem. 2020 Nov;412(28):7861-7869. doi: 10.1007/s00216-020-02918-9. Epub 2020 Sep 1. PMID: 32870352; PMCID: PMC7461152.