LDS-sample buffer (4X), non-reducing is used in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). It is used in denaturing gels and is compatible with both coomassie and silver staining and Western blotting applications.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Applications
LDS-sample buffer (4X), non-reducing is used in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). It is used in denaturing gels and is compatible with both coomassie and silver staining and Western blotting applications.
Solubility
Not miscible in water.
Notes
This buffer contains: 988mM Tris-HCl, 2.04mM EDTA, 8% LDS, 40% glycerol, 0.88% Coomassie blue and 0.7mM phenol red at pH 8.5.
RUO – Research Use Only
Yi Wang; Qiaozhen Lu; Shiaw-Lin Wu; Barry L. Karger and William S. Hancock. Characterization and Comparison of Disulfide Linkages and Scrambling Patterns in Therapeutic Monoclonal Antibodies: Using LC-MS with Electron Transfer Dissociation. Anal. Chem. 2011, 83, (8), 3133-3140.
Dan Lu; Zhenping Zhu. Construction and Production of an IgG-Like Tetravalent Bispecific Antibody for Enhanced Therapeutic Efficacy. Therapeutic Antibodies. 2008, 525, 377-404.