Trichloroacetic acid, is traditionally used to precipitate protein. Has been used to determine protein concentration by quantitative precipitation. It is also used as a decalcifier and fixative in microscopy in protein sequencing, detecting albumin and organic synthesis. A protocol for the precipitation of nucleic acids can be found in Molecular Cloning.
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Applications
Trichloroacetic acid, is traditionally used to precipitate protein. Has been used to determine protein concentration by quantitative precipitation. It is also used as a decalcifier and fixative in microscopy in protein sequencing, d
RUO – Research Use Only
General References:
- RW Blakesley.; JA Boezi. A new staining technique for proteins in polyacrylamide gels using Coomassie Brilliant Blue G250. Analytical biochemistry. 1977, 82(2), 580-582.
- WH Marsh.; B Fingerhut.; H Miller. Automated and manual direct methods for the determination of blood urea. Clinical chemistry. 1965, 11(6), 624-627.