It is used as an alkaline transfer buffer used for the transfer of strongly basic proteins from gels to nitrocellulose.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Applications
It is used as an alkaline transfer buffer used for the transfer of strongly basic proteins from gels to nitrocellulose.
Solubility
Soluble in water (333.33 mg/ml, clear to slightly hazy colorless solution).
Notes
Store in cool place. Keep container tightly closed in a dry and well-ventilated place. Keep away from strong oxidizing agents.
RUO – Research Use Only
T Thiel.; L Liczkowski.; ST Bissen. New zwitterionic butanesulfonic acids that extend the alkaline range of four families of good buffers: Evaluation for use in biological systems. Journal of Biochemical and Biophysical Methods. 1998, 37(3), 117-129.
D Tan.; MJ Barber.; GC Ferreira. The role of tyrosine 121 in cofactor binding of 5-aminolevulinate synthase. Protein science. 1998, 7(5), 1208-1213.