Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
General Description
• Streptavidin is a 60-kDa extracellular protein isolated from Streptomyces avidinii, and it is considered a tetrameric protein with a very high affinity to biotin
• It has four binding sites for biotin and has the ability to bind four molecules of biotin
Application
• This compound has a strong affinity for biotin and biotinylated molecules, and because of this, streptavidin is considered one of the most important components in laboratory research kits. Due to its affinity for biotin, it is widely used in several molecular biology techniques, including ELISA, DNA purification, enzyme immobilization, and flow cytometry
• Streptavidin is used to visualize biotin-conjugated molecules in enzyme-linked immunosorbent assay (ELISA)
• Streptavidin has been used in microbubble preparation, which is an important technique used in research
• It has been used extensively in bionanotechnology to prepare beads and nanoparticles
• It is used to immobilize proteins and oligonucleotides
Literature References
Lu, X.; Wu, D.; Li, Z.; Chen, G. Chapter 7 - Polymer Nanoparticles. Progress in Molecular Biology and Translational Science. 2011, 104, 299-323.
Rooij, T.; Skachkov, S.; Beekers, I.; et al. Viability of endothelial cells after ultrasound-mediated sonoporation: Influence of targeting, oscillation, and displacement of microbubbles. J Control Release. 2016, 238, 197-211.
Meyer, D.L., et al. Reduced antibody response to streptavidin through site-directed mutagenesis. Protein Sci. 2001, 10, (3), 491-503.
Sabanayagam C, Smith C, Cantor C. Oligonucleotide immobilization on micropatterned streptavidin surfaces. Nucleic Acids Res. 2000, 28, (8), E33.
Rusakiewicz, S., et al. Soluble HLA/peptide monomers cross-linked with co-stimulatory antibodies onto a streptavidin core molecule efficiently stimulate antigen-specific T cell responses. Cancer Immunol. Immunother. 2009, 58, (9), 1459-1470.