HEPES-buffered saline, pH 7.0 (2X for transfection)
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HEPES-buffered saline, pH 7.0 (2X for transfection)
Thermo Scientific Chemicals

HEPES-buffered saline, pH 7.0 (2X for transfection)

HEPES-buffered saline (2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonic acid, CAS # 7365-45-9) is a Good’s buffer used to maintain physiological pH.
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Catalog number J62623.AP
also known as J62623-AP
Price (USD)/ Each
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Quantity:
500 mL
Request bulk or custom format
Price (USD)/ Each
153.65
Special Offer
Online exclusive
Ends: 31-Dec-2024
181.00 
Save 27.35 (15%)
Add to cart
HEPES-buffered saline, pH 7.0 (2X for transfection)
Catalog numberJ62623.AP
Price (USD)/ Each
153.65
Special Offer
Online exclusive
Ends: 31-Dec-2024
181.00 
Save 27.35 (15%)
-
Add to cart
Chemical Identifiers
CAS7365-45-9
IUPAC Name2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid
Molecular FormulaC8H18N2O4S
InChI KeyJKMHFZQWWAIEOD-UHFFFAOYSA-N
SMILESOCCN1CCN(CCS(O)(=O)=O)CC1
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SpecificationsSpecification SheetSpecification Sheet
CommentAll components are dissolved in UV treated 18.2 megohm-cm ± 1 water, followed by pH adjustment and filtered through 0.22 micron filter.
Appearance (Color)Clear colorless
FormLiquid
pH7.0 ± 0.2 (1X)
HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological pH. It acts a buffering component, which is used in the preparation of buffers. It is described as one of the best all-purpose buffers available for use in biological research.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

  • HEPES-buffered saline is an ionic buffer with a pKa=7.55
  • HEPES is an ethanesulfonic acid with a 4-(2-hydroxyethyl)piperazin-1-yl substitution within one of its methyl groups

Application

  • HEPES-buffered saline can be used to maintain physiological pH in a solution; it is frequently used for protein isolation and other molecular applications
  • Useful for cell culture, HEPES maintains pH even in the presence of carbon dioxide
  • It is intended for use in cell culture and molecular biology
RUO – Research Use Only

General References:

  1. Rochat, S.; Alberio, L. Formaldehyde-fixation of platelets for flow cytometric measurement of phosphatidylserine exposure is feasible. Cytometry A 2015, 87 (1), 32-36.
  2. Yang, H. N.; Park, J. S.; Jeon, S. Y.; Park, K. H. Carboxymethylcellulose (CMC) formed nanogels with branched poly (ethyleneimine)(bPEI) for inhibition of cytotoxicity in human MSCs as a gene delivery vehicles. Carbohydr. Polym. 2015, 122, 265-275.