Sodium citrate, 0.5M buffer soln., pH 5.0
Sodium citrate, 0.5M buffer soln., pH 5.0
Thermo Scientific Chemicals

Sodium citrate, 0.5M buffer soln., pH 5.0

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Quantity:
500 mL
250 mL
Catalog number J62918.AP
also known as J62918-AP
Price (USD)/ Each
113.65
Online exclusive
126.00 
Save 12.35 (10%)
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Quantity:
500 mL
Request bulk or custom format
Price (USD)/ Each
113.65
Online exclusive
126.00 
Save 12.35 (10%)
Add to cart
Sodium citrate, 0.5M buffer soln., pH 5.0
Catalog numberJ62918.AP
Price (USD)/ Each
113.65
Online exclusive
126.00 
Save 12.35 (10%)
-
Add to cart
Specifications
Chemical Name or MaterialSodium citrate buffer soln.
Health Hazard 1Danger
ColorColorless
Health Hazard 2GHS H Statement
H318
Causes serious eye damage.
Health Hazard 3GHS P Statement
P280-P305+P351+P338-P310
Wear protective gloves/protective clothing/eye protection/face protection.
IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor/physician.
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Citrate, is used as an anticoagulant and also used as a biological buffer. It is often used to prepare sodium citrate buffer for antigen retrieval of tissue samples. The citrate solution is designed to break protein cross-links, thus, unmasking antigens and epitopes in formalin-fixed and paraffin embedded tissue sections, resulting in enhancing staining intensity of antibodies. Citrate has anticoagulant activity and as a calcium chelator, it forms complexes that disrupt the tendency of blood to clot.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
Citrate, is used as an anticoagulant and also used as a biological buffer. It is often used to prepare sodium citrate buffer for antigen retrieval of tissue samples. The citrate solution is designed to break protein cross-links, thus, unmasking antigens and epitopes in formalin-fixed and paraffin embedded tissue sections, resulting in enhancing staining intensity of antibodies. Citrate has anticoagulant activity and as a calcium chelator, it forms complexes that disrupt the tendency of blood to clot.

Solubility
It is soluble in water (29.4 g/L) at 20°C. Insoluble in ethanol.

Notes
Store in cool place. Keep container tightly closed in a dry and well-ventilated place. Keep away from strong oxidizing agents.
RUO – Research Use Only

General References:

  1. YP Lee.; T Takahashi. An improved colorimetric determination of amino acids with the use of ninhydrin. Analytical Biochemistry. 1972, 14,(1), 71-77.
  2. XZ Shu.; KJ Zhu.; W Song. Novel pH-sensitive citrate cross-linked chitosan film for drug controlled release. International Journal of Pharmaceutics. 2001, 212,(1), 19-28.