Arsenazo III, Thermo Scientific Chemicals
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Arsenazo III, Thermo Scientific Chemicals
Thermo Scientific Chemicals

Arsenazo III, Thermo Scientific Chemicals

Arsenazo III (3,6-bis((E)-(2-Arsonophenyl)diazenyl)-4,5-dihydroxynaphthalene-2,7-disulfonic acid, CAS # 1668-00-4) is a metallochrome indicator that is used for detecting calcium levels in plasma and serum.
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Catalog number J63031.14
also known as J63031-14
Price (USD)/ Each
673.65
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Ends: 31-Dec-2024
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Quantity:
25 g
Request bulk or custom format
Price (USD)/ Each
673.65
Special Offer
Online exclusive
Ends: 31-Dec-2024
792.00 
Save 118.35 (15%)
Add to cart
Arsenazo III, Thermo Scientific Chemicals
Catalog numberJ63031.14
Price (USD)/ Each
673.65
Special Offer
Online exclusive
Ends: 31-Dec-2024
792.00 
Save 118.35 (15%)
-
Add to cart
Chemical Identifiers
CAS1668-00-4
IUPAC Name3,6-bis[2-(2-arsonophenyl)hydrazin-1-ylidene]-4,5-dioxo-3,4,5,6-tetrahydronaphthalene-2,7-disulfonic acid
Molecular FormulaC22H18As2N4O14S2
InChI KeyTVMZRHVOFZTNET-UHFFFAOYSA-N
SMILESO[As](O)(=O)C1=CC=CC=C1NN=C1C(=O)C2=C(C=C1S(O)(=O)=O)C=C(C(=NNC1=CC=CC=C1[As](O)(O)=O)C2=O)S(O)(=O)=O
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SpecificationsSpecification SheetSpecification Sheet
FormCrystals or powder or crystalline powder
Appearance (Color)Dark green to dark brown to dark red to black
Assay from Suppliers CofA≥70%
Loss on Drying≤12.0% (200°C/constant weight)
lambda-max (UV-Vis)528-545nm
Suitable for determination of micromolar amounts of calcium

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

• Arsenazo III is highly specific for calcium and produces absorbance peaks in the 600-660 nm range
• It reacts in slightly acidic conditions, and is soluble in alcohol and semi-soluble in water

Application

• Arsenazo III can detect calcium in permeabilized cells; it is also capable of detecting uranium and thorium
• Arsenazo III has been used in vitro to measure intracellular Ca2+ levels in live neurons alongside patch clamp methods

RUO – Research Use Only

General References:

  1. Yadav R, Pandey BN, Kumar A. Estimation and in-situ detection of thorium in human liver cell culture by arsenazo-III based colorimetric assay. Biometals. 2020 Feb;33(1):75-85. doi: 10.1007/s10534-019-00231-2. Epub 2020 Jan 2. PMID: 31897857.
  2. Hogendoorn C, Roszczenko-Jasińska P, Martinez-Gomez NC, de Graaff J, Grassl P, Pol A, Op den Camp HJM, Daumann LJ. Facile Arsenazo III-Based Assay for Monitoring Rare Earth Element Depletion from Cultivation Media for Methanotrophic and Methylotrophic Bacteria. Appl Environ Microbiol. 2018 Apr 2;84(8):e02887-17. doi: 10.1128/AEM.02887-17. PMID: 29453257; PMCID: PMC5881054.