LB Agar plate with 100μg/mL Ampicillin

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

• LB agar plate is a nutrient-rich media frequently used to culture bacteria in the lab, including Escherichia coli

Application

• LB agar plate is used to culture bacteria in microbiology research. These plates are commonly used during cloning experiments to amplify antibiotic-resistant, competent bacteria
• The addition of agar to LB medium helps form a gel that allows bacteria to grow. The bacteria cannot digest the agar but can obtain the needed nutrients to grow from the LB medium
• The addition of ampicillin (100 μg/mL) to these plates allows selection of the bacteria that can grow in the presence of ampicillin which is conferred by a plasmid carrying the antibiotic resistance gene