Deoxyribonucleic acid sodium salt, is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard to determine DNA content in gels and for DNA quantification by fluorescent assay.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Applications
Deoxyribonucleic acid sodium salt, is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard to determine DNA content in gels and for DNA quantification by fluorescent assay.
Solubility
Soluble in water.
Notes
Highly polymerized. Hyperchromicity is at least 27%.
RUO – Research Use Only
General References:
- K. Burton. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956, 62 (2), 315-323.
- Peter W.J. Rigby.; Marianne Dieckmann.; Carl Rhodes.; Paul Berg. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I . Journal of Molecular Biology. 1997, 113 (1), 237-251.