Search Thermo Fisher Scientific
Element Search
Structure Search
Thermo Scientific Chemicals
Guanidine hydrochloride, Molecular Biology Grade
A protein denaturant
Have Questions?
Change view
Catalog number J65661.22
also known as J65661-22
Price (USD)/ Each
80.65
Online exclusive
89.30 Save 8.65 (10%)
-
Quantity:
100 g
Price (USD)/ Each
80.65
Online exclusive
89.30 Save 8.65 (10%)
Guanidine hydrochloride, Molecular Biology Grade
Catalog numberJ65661.22
Price (USD)/ Each
80.65
Online exclusive
89.30 Save 8.65 (10%)
-
Chemical Identifiers
CAS50-01-1
IUPAC Namehydrogen guanidine chloride
Molecular FormulaCH6ClN3
InChI KeyPJJJBBJSCAKJQF-UHFFFAOYSA-N
SMILES[H+].[Cl-].NC(N)=N
View more
Specifications
Specification Sheet
Specification SheetAssay (unspecified)Absorbance (260nm, 6M in water): 0.03 max.
Appearance (Color)White
FormPowder
Assay (Titration)99.5% min.
DNaseNone detected
View more
Guanidine hydrochloride is used for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Applications
Guanidine hydrochloride is used for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase.
Solubility
Freely soluble in water
Notes
Protect from heat. Store away from oxidizing agents.
Guanidine hydrochloride is used for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase.
Solubility
Freely soluble in water
Notes
Protect from heat. Store away from oxidizing agents.
RUO – Research Use Only
General References:
- Dipti Arora.; Navin Khanna. Method for increasing the yield of properly folded recombinant human gamma interferon from inclusion bodies. Journal of Biotechnology. 1996, 52, (2), 127-133.
- Cing-Yuen Won.; Maurice R. Eftin. Incorporation of Tryptophan Analogues into Staphylococcal Nuclease, Its V66W Mutant, and Δ137-149 Fragment: Spectroscopic Studies. Biochemistry. 1998, 37, (25), 8938-8946.