Nisin, 900 I.U./mg, Thermo Scientific Chemicals
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Nisin, 900 I.U./mg, Thermo Scientific Chemicals
Thermo Scientific Chemicals

Nisin, 900 I.U./mg, Thermo Scientific Chemicals

Nisin, CAS # 1414-45-5, is a polycyclic peptide derived from and produced by Lactococcus lactis that shows antibacterial activity against gram-positive bacteria.
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Catalog number J66370.14
also known as J66370-14
Price (USD)/ Each
458.65
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Ends: 31-Dec-2024
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Quantity:
25 g
Request bulk or custom format
Price (USD)/ Each
458.65
Special Offer
Online exclusive
Ends: 31-Dec-2024
539.00 
Save 80.35 (15%)
Add to cart
Nisin, 900 I.U./mg, Thermo Scientific Chemicals
Catalog numberJ66370.14
Price (USD)/ Each
458.65
Special Offer
Online exclusive
Ends: 31-Dec-2024
539.00 
Save 80.35 (15%)
-
Add to cart
Chemical Identifiers
CAS1414-45-5
IUPAC Name6-amino-2-{2-[2-(2-{2-[2-({7-[6-amino-2-(2-{2-[(21-{6-amino-2-[(10-{15-[(2Z)-2-(2-amino-3-methylpentanamido)but-2-enamido]-12-(butan-2-yl)-9-methylidene-6-(2-methylpropyl)-5,8,11,14-tetraoxo-1-thia-4,7,10,13-tetraazacyclohexadecane-3-amido}-9-methyl-1,4,11-trioxo-tetradecahydropyrrolo[2,1-i]1-thia-4,7,10-triazacyclotridecan-6-yl)formamido]hexanamido}-15,22-dimethyl-12-(2-methylpropyl)-9-[2-(methylsulfanyl)ethyl]-5,8,11,14,17,20-hexaoxo-1-thia-4,7,10,13,16,19-hexaazacyclodocosan-3-yl)formamido]-3-carbamoylpropanamido}-4-(methylsulfanyl)butanamido)hexanamido]-14-[(1H-imidazol-5-yl)methyl]-4,8,20-trimethyl-3,6,12,15,21-pentaoxo-9,19-dithia-2,5,13,16,22-pentaazabicyclo[9.9.2]docosan-17-yl}formamido)-3-hydroxypropanamido]-3-methylpentanamido}-3-(1H-imidazol-5-yl)propanamido)-3-methylbutanamido]prop-2-enamido}hexanoic acid
Molecular FormulaC143H230N42O37S7
InChI KeyNVNLLIYOARQCIX-GSJOZIGCNA-N
SMILESCCC(C)C(N)C(=O)NC(=C/C)C(=O)NC1CSCC(NC(=O)C(CC(C)C)NC(=O)C(=C)NC(=O)C(NC1=O)C(C)CC)C(=O)NC1C(C)SCC(NC(=O)CNC(=O)C2CCCN2C1=O)C(=O)NC(CCCCN)C(=O)NC1C(C)SCC(NC(=O)CNC(=O)C(CCSC)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)CNC1=O)C(=O)NC(CC(N)=O)C(=O)NC(CCSC)C(=O)NC(CCCCN)C(=O)NC1C(C)SCC2NC(=O)C(NC(=O)C(C)NC1=O)C(C)SCC(NC(=O)C(CC1=CN=CN1)NC2=O)C(=O)NC(CO)C(=O)NC(C(C)CC)C(=O)NC(CC1=CN=CN1)C(=O)NC(C(C)C)C(=O)NC(=C)C(=O)NC(CCCCN)C(O)=O
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SpecificationsSpecification SheetSpecification Sheet
Appearance (Color)Pale cream to pale brown
Assay from Suppliers CofASodium Chloride: ≥50.0%
FormPowder
Assay from Suppliers CofATiter: ≥900 IU/mg
Loss on Drying≤5.0%
Nisin, 900 I.U./mg is commonly used as a food preservative. It is also used as a selective agent in cell culture for the isolation of gram-negative bacteria, yeast, and molds. Nisin has been used in bioassays to study posttranslational modification. It binds and interferes with the permeability of the cytoplasmic membrane.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description
• Nisin is a broad-spectrum polycyclic peptide derived from and produced by Lactococcus lactis that shows antibacterial activity against gram-positive bacteria
Nisin binds lipid II in the cell membrane. After binding it can insert into the membrane, creating a pore
Nisin can efficiently inhibit the growth of gram-positive bacteria, and resistance is rarely observed
Application
• Nisin is commonly used as a selective compound in cell culture to isolate gram-negative bacteria, yeast, and molds (a type of fungus)
• It has been used in bioassays to study posttranslational modification
• This compound has the ability to bind and interfere with the permeability of the cytoplasmic membrane
• It is used to study the assembly and stability of nisin-lipid II pore complexes
• This compound is used in in vitro experiments to form targeted pores in cell membranes that are mediated by lipid II
RUO – Research Use Only

General References:

  1. Olli Koponen; Marja Tolonen; Mingqiang Qiao; Gudrun Wahlström; Jari Helin; Per E J Saris. NisB is required for the dehydration and NisC for the lanthionine formation in the post-translational modification of nisin. Microbiology.2002, 148, (Pt 11), 3561-3568.
  2. Brötz H.; and Sahl H. New insights into the mechanism of action of lantibiotics - Diverse biological effects by binding to the same molecular target. J Antimicrob Chemother.2000, 46, (1), 1-6.
  3. Hasper HE, de Kruijff B, Breukink E. Assembly and stability of nisin-lipid II pores. Biochemistry. 2004, 43, (36), 11567-11575.
  4. Wiedemann I, Benz R, Sahl HG. Lipid II-mediated pore formation by the peptide antibiotic nisin: a black lipid membrane study. J Bacteriol. 2004, 186, (10), 3259-3261.
  5. Zhou L, van Heel AJ, Montalban-Lopez M, Kuipers OP. Potentiating the Activity of Nisin against Escherichia coli. Front Cell Dev Biol. 2016, 4, 7.
  6. Olli Koponen; Marja Tolonen; Mingqiang Qiao; Gudrun Wahlström; Jari Helin; Per E J Saris. NisB is required for the dehydration and NisC for the lanthionine formation in the post-translational modification of nisin. Microbiology. 2002, 148, (Pt 11), 3561-3568.
  7. Brötz H.; and Sahl H. New insights into the mechanism of action of lantibiotics - Diverse biological effects by binding to the same molecular target. J Antimicrob Chemother. 2000, 46 (1), 1-6.