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N,N'-Bis(acryloyl)cystamine, Electrophoresis Reagent, 98%, Thermo Scientific Chemicals
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Catalog number J66893.06
also known as J66893-06
Price (USD)/ Each
222.65
Online exclusive
247.00 Save 24.35 (10%)
-
Quantity:
5 g
Price (USD)/ Each
222.65
Online exclusive
247.00 Save 24.35 (10%)
N,N'-Bis(acryloyl)cystamine, Electrophoresis Reagent, 98%, Thermo Scientific Chemicals
Catalog numberJ66893.06
Price (USD)/ Each
222.65
Online exclusive
247.00 Save 24.35 (10%)
-
Chemical Identifiers
CAS60984-57-8
IUPAC NameN-(2-{[2-(prop-2-enamido)ethyl]disulfanyl}ethyl)prop-2-enamide
Molecular FormulaC10H16N2O2S2
InChI KeyDJVKJGIZQFBFGS-UHFFFAOYSA-N
SMILESC=CC(=O)NCCSSCCNC(=O)C=C
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Specifications
Specification Sheet
Specification SheetFormPowder
Appearance (Color)White
Assay from Supplier's CofA≥97.5%
Melting Point121-126°C
BAC is used as a reversible cross-linker for polyacrylamide gels, to simplify isolation of macromolecules separated on the gels. The disulfide linkage can be broken with a suitable reducing agent. A 1-g slice of a 4% (12:1) gel will dissolve in 5 mL of 100 mM DTT in TBE (pH 8.3) in less than 30 min. The pH of 8.3 is optimum for rapid dissolution of the gel.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Applications
BAC is used as a reversible cross-linker for polyacrylamide gels, to simplify isolation of macromolecules separated on the gels. The disulfide linkage can be broken with a suitable reducing agent. A 1-g slice of a 4% (12:1) gel will dissolve in 5 mL of 100 mM DTT in TBE (pH 8.3) in less than 30 min. The pH of 8.3 is optimum for rapid dissolution of the gel.
Solubility
Soluble at 50 mg/ml in acetic acid
Notes
Store at -20°C. Incompatible with heat, oxidizing agents.
BAC is used as a reversible cross-linker for polyacrylamide gels, to simplify isolation of macromolecules separated on the gels. The disulfide linkage can be broken with a suitable reducing agent. A 1-g slice of a 4% (12:1) gel will dissolve in 5 mL of 100 mM DTT in TBE (pH 8.3) in less than 30 min. The pH of 8.3 is optimum for rapid dissolution of the gel.
Solubility
Soluble at 50 mg/ml in acetic acid
Notes
Store at -20°C. Incompatible with heat, oxidizing agents.
RUO – Research Use Only
General References:
- Haruyuki Hiratani.; Carmen Alvarez-Lorenzo.; Jeffrey Chuang.; Orhan Guney.; Alexander Yu. Grosberg.; Toyoichi Tanaka. Effect of Reversible Cross-linker, N,N‘-Bis(acryloyl)cystamine, on Calcium Ion Adsorption by Imprinted Gels.Langmuir. 2001, 17 (14), 4431-4436 .
- Hua Yu.; David W. Grainger. Amphiphilic Thermosensitive N-Isopropylacrylamide Terpolymer Hydrogels Prepared by Micellar Polymerization in Aqueous Media.Macromolecules . 1994, 27 (16),4554-4560 .