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Thermo Scientific™
PCR Master Mix (2X)
Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers.
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| Catalog Number | No. of Reactions |
|---|---|
| K0171 | 200 Reactions |
| K0172 | 1000 Reactions |
Catalog number K0171
Price (USD)
167.65
Online Exclusive
195.00Save 27.35 (14%)
Each
In stock
No. of Reactions:
200 Reactions
Price (USD)
167.65
Online Exclusive
195.00Save 27.35 (14%)
Each
Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR.
Highlights
- Convenient, ready-to-use mix
- Thermostable—half life is more than 40 min. at 95°C
- Generates PCR products with 3'-dA overhangs
- Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)
Applications
- Routine PCR amplification of DNA fragments up to 5 kb
- High throughput PCR
- DNA labeling
Note
The error rate of Taq DNA Polymerase in PCR is 2.2 x 10-5 errors per nt per cycle, as determined by a modified method described in. Accordingly, the accuracy of PCR is 4.5 x 104. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration2X
Fidelity (vs. Taq)1X
Hot StartNo
No. of Reactions200 Reactions
Overhang3'-A
PolymeraseTaq DNA Polymerase
Product TypePCR Master Mix
Quantity200 reactions
Reaction FormatSuperMix or Master Mix
Starting MaterialDNA
For Use With (Application)Standard PCR
GC-Rich PCR PerformanceLow
Reaction SpeedStandard
Unit SizeEach
Contents & Storage
• 2X PCR Master Mix, 4 x 1.25 mL
• Nuclease-free water, 4 x 1.25 mL
Store at -20°C.
• Nuclease-free water, 4 x 1.25 mL
Store at -20°C.