PCR Master Mix (2X)
PCR Master Mix (2X)
Thermo Scientific™

PCR Master Mix (2X)

Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers.
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Catalog NumberNo. of Reactions
K01721000 Reactions
K0171200 Reactions
Catalog number K0172
Price (USD)
686.65
Online Exclusive
800.00
Save 113.35 (14%)
Each
In stock
Add to cart
No. of Reactions:
1000 Reactions
Request bulk or custom format
Price (USD)
686.65
Online Exclusive
800.00
Save 113.35 (14%)
Each
Add to cart
Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR.

Highlights

  • Convenient, ready-to-use mix
  • Thermostable—half life is more than 40 min. at 95°C
  • Generates PCR products with 3'-dA overhangs
  • Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)

Applications

  • Routine PCR amplification of DNA fragments up to 5 kb
  • High throughput PCR
  • DNA labeling

Note

The error rate of Taq DNA Polymerase in PCR is 2.2 x 10-5 errors per nt per cycle, as determined by a modified method described in. Accordingly, the accuracy of PCR is 4.5 x 104. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration2X
Fidelity (vs. Taq)1X
Hot StartNo
No. of Reactions1000 Reactions
Overhang3'-A
PolymeraseTaq DNA Polymerase
Product TypePCR Master Mix
Quantity1,000 reactions
Reaction FormatSuperMix or Master Mix
Shipping ConditionDry Ice
Starting MaterialDNA
For Use With (Application)Standard PCR
GC-Rich PCR PerformanceLow
Reaction SpeedStandard
Unit SizeEach
Contents & Storage
• 2X PCR Master Mix, 20 x 1.25 mL
• Nuclease-free water, 20 x 1.25 mL

Store at -20°C.
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Frequently asked questions (FAQs)

Can I do PCR directly from bacterial colonies with Thermo Scientific Taq Master Mixes?

Dream Taq PCR Master Mix and Taq PCR Master Mix can be used for direct PCR from bacterial colonies. Simply scrape a tiny amount of a single colony and transfer to a PCR-tube, prefilled with the master mix. Always use at least 3 min at 95 degrees C for initial denaturation.

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