GeneJET Gel Extraction Kit
We have updated the storage temperature of the purification columns for this product. For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C.
GeneJET Gel Extraction Kit
Thermo Scientific™

GeneJET Gel Extraction Kit

Thermo Scientific GeneJET Gel Extraction Kit is designed for rapid and efficient purification of DNA fragments from standard or low-meltingRead more
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Catalog NumberQuantity
K069150 Preps
K0692250 Preps
Catalog number K0691
Price (USD)
93.65
Online Exclusive
117.00
Save 23.35 (20%)
Each
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Quantity:
50 Preps
Price (USD)
93.65
Online Exclusive
117.00
Save 23.35 (20%)
Each
Add to cart
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Thermo Scientific GeneJET Gel Extraction Kit is designed for rapid and efficient purification of DNA fragments from standard or low-melting point agarose gels run in either TAE or TBE buffer.

The kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column. The kit can be used to purify DNA fragments from 25 bp to 20 kb in size with recovery rates up to 95%. Each GeneJET purification column has a binding capacity of up to 25 μg of DNA and can process up to 1 g of agarose gel. The entire procedure takes just 15 minutes, and the isolated DNA is ready to use in all common downstream applications, including ligation, restriction digestion, PCR, sequencing, and labeling.

Highlights

  • Highly efficient: up to 95% recoveries in the range of 100 bp to 10 kb
  • Fast: procedure takes only 15 minutes
  • Convenient: spin columns are capped and assembled with collection tubes

Applications

Fast and efficient extraction of high purity DNA fragments ideal for use in all conventional molecular biology procedures including:

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Column TypeSpin Column
For Use With (Application)Real-Time Quantitative PCR (qPCR), Cloning, Southern Blotting, Nucleic Acid Labeling, Sequencing
High-throughput CompatibilityNot High-throughput Compatible (Manual)
Product LineGeneJET
Product TypeGel Extraction Kit
Quantity50 Preps
Sample TypeGel
TargetDNA from Enzymatic Reactions (e.g. PCR)
Test Time15 min.
FormatSpin Column
Isolation TechnologySpin Column
Unit SizeEach
Contents & Storage
For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C.
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Frequently asked questions (FAQs)

Can I use water instead of Elution Buffer in the elution step of GeneJET and MagJET nucleic acid purification kits?

We would highly recommend using the provided Elution Buffer in the elution step for best results. You may use nuclease-free water; however, it may affect the yield of the nucleic acids during purification and their stability during storage.

Can I use a vacuum manifold with GeneJET kits?

The GeneJET spin columns are compatible with vacuum manifolds of standard size (e.g. Qiagen, Sigma-Aldrich, Promega). However, not all GeneJET kits are supported with a vacuum protocol. Please refer to the particular kit protocol for the detailed procedure.

Can I use GeneJET Gel Extraction Kit for PCR clean-up?

The GeneJET Gel Extraction Kit is designed for gel extraction but can also be used for PCR clean-up by following the protocol from the GeneJET PCR Purification Kit. The components in the GeneJET Gel Extraction Kit and GeneJET PCR Purification Kit are very similar and can be used interchangeably. The exact composition of solutions in the kits is proprietary.

For a 3-in-1 kit for purification of PCR products from enzymatic reaction mixtures and agarose gels, we recommend using the GeneJET Gel Extraction and DNA Cleanup Micro Kit (Cat. Nos. K0831/2).

At what temperature should I perform centrifugation steps in the GeneJET kits?

We recommend performing centrifugation steps at room temperature when the temperature is not specified.

Are GeneJET spin columns or MagJET magnetic beads available separately?

No, we do not offer GeneJET columns or MagJET magnetic beads separately at this time.

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