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S.N.A.P.™ Plasmid DNA MiniPrep Kit
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Invitrogen™

S.N.A.P.™ Plasmid DNA MiniPrep Kit

The S.N.A.P.™ MiniPrep Kit is a plasmid DNA purification system designed to yield pure plasmid DNA (Figures 1 and 2)Read more
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Catalog NumberQuantity
K190001100 preps
Catalog number K190001
Price (USD)
612.00
Each
Add to cart
Quantity:
100 preps
Price (USD)
612.00
Each
Add to cart
The S.N.A.P.™ MiniPrep Kit is a plasmid DNA purification system designed to yield pure plasmid DNA (Figures 1 and 2) for automated sequencing in just 25 minutes. Pure plasmid DNA can improve your automated sequencing results by producing:

• Longer reads
• Lower background
• Higher accuracy
• Stronger signal peaks

To ensure the highest quality, each lot of S.N.A.P.™ MiniPrep Kits is used in an automated sequencing reaction. At least 450 bases of 98.5% accurate sequence must be obtained. Typical read lengths are as high as 650 bases. The S.N.A.P.™ MiniPrep Kit yields up to 10 μg of pure miniprep plasmid DNA from 1-3 ml of overnight culture.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Final Product TypePlasmid DNA
For Use With (Application)Next-Generation Sequencing, In Vitro Transcription, Sequencing, Cloning, Transformation
High-throughput CompatibilityNot High-throughput Compatible (Manual)
No. of Reactions100 preps
Prep Scale< 100 μg (Small-Scale) Plasmid DNA
Product LineS.N.A.P. ™
Product TypePlasmid DNA MiniPrep Kit
Quantity100 preps
Sample TypeBacterial Culture
TargetPlasmid DNA
Test Time25 min.
FormatKit
Isolation TechnologySpin Column
Unit SizeEach
Contents & Storage
Store the S.N.A.P.™ MiniPrep spin columns, lysis buffer, binding buffer, wash buffer, 4X final wash, and the collection tubes at room temperature. Store the resuspension buffer, precipitation salt solution, and RNase A at +4°C. All reagents are guaranteed stable for 6 months when properly stored.

Frequently asked questions (FAQs)

What is the upper size limit for plasmid purification with the S.N.A.P. Miniprep Kit?

Bacmid DNA (>100 kbp) has been purified with the S.N.A.P. miniprep kit.

The PureLink HiPure Plasmid DNA Purification Kits (mini/midi/maxi scales) can also be used for large constructs. The HiPure kit manuals contain a modified protocol designed to help increase yields with these large plasmid isolations.

What are the compositions of the S.N.A.P. MiniPrep and MidiPrep Kit solutions?

The formulations are published in the manual for the S.N.A.P. MidiPrep kit, and are the same as the ones used in the MiniPrep kit.

What is the resin in the S.N.A.P. kits?

The column contains a silica/glass fiber based resin. However, the exact composition is proprietary.

Why is LB medium recommended for growing up plasmid preps in the S.N.A.P. protocol?

The volumes of culture recommended in our S.N.A.P. kits assume an OD of no more than 2.0. If a rich medium is used, the volume processed should be reduced to avoid overloading the column.

Why can't I dissolve all of my DNA after precipitation?

Sometimes there is insoluble material (probably resin fines) in the precipitated DNA. This material is very easily removed with a 10 second spin. The presence of these particles does not reflect a poor preparation. Once the particles are removed, the DNA performs well in all applications.

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