Zero Blunt™ TOPO™ PCR Cloning Kit, with pCR™-Blunt II-TOPO™ Vector, One Shot™ TOP10 Chemically Competent E. coli, and PureLink™ Quick Plasmid Miniprep Kit
Zero Blunt&trade; TOPO&trade; PCR Cloning Kit, with pCR&trade;-Blunt II-TOPO&trade; Vector, One Shot&trade; TOP10 Chemically Competent <i>E. coli</i>, and PureLink&trade; Quick Plasmid Miniprep Kit
Invitrogen™

Zero Blunt™ TOPO™ PCR Cloning Kit, with pCR™-Blunt II-TOPO™ Vector, One Shot™ TOP10 Chemically Competent E. coli, and PureLink™ Quick Plasmid Miniprep Kit

Zero Blunt™ TOPO™ PCR Cloning Kits offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplifiedRead more
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Catalog number K280002
Price (USD)/ Each
854.65
Online exclusive
918.00 
Save 63.35 (7%)
-
Add to cart
Quantity:
25 Reactions
Price (USD)/ Each
854.65
Online exclusive
918.00 
Save 63.35 (7%)
Add to cart
Zero Blunt™ TOPO™ PCR Cloning Kit, with pCR™-Blunt II-TOPO™ Vector, One Shot™ TOP10 Chemically Competent E. coli, and PureLink™ Quick Plasmid Miniprep Kit
Catalog numberK280002
Price (USD)/ Each
854.65
Online exclusive
918.00 
Save 63.35 (7%)
-
Add to cart
Zero Blunt™ TOPO™ PCR Cloning Kits offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases. The kits include linearized and topoisomerase I-activated pCR™-Blunt II-TOPO™ Vector for 5-minute benchtop ligations without ligase (1). The pCR™-Blunt II-TOPO™ Vector also features:

ccdB gene for positive selection (2)
EcoR I sites flanking the PCR product insertion site for easy excision of inserts
• Kanamycin and Zeocin™ resistance genes for your choice of selection in E. coli
• SP6 promoter/primer site for in vitro RNA transcription and sequencing
• M13 forward and reverse primer sites for sequencing or PCR screening

Selected Zero Blunt™ TOPO™ PCR Cloning Kits are available in combo kits combined with a PureLink™ Quick Plasmid Miniprep Kit (50 preps) for fast plasmid purification of your TOPO™-cloned inserts for downstream analysis.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Bacterial or Yeast StrainTOP10
Cell TypeChemically Competent
Cloning MethodBlunt TOPO™
FormatKit
Product LineOne Shot™
Product TypePCR Cloning Kit
PromoterSP6
Quantity25 Reactions
VectorBlunt DNA Cloning Vectors
Unit SizeEach
Contents & Storage
Box 1:
• Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
1x1 image pixel for data collection