NT-GFP Fusion TOPO™ Expression Kit
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Invitrogen™

NT-GFP Fusion TOPO™ Expression Kit

The GFP Fusion TOPO™ TA Expression Kits are designed to allow fusion of a protein of interest to the Cycle자세히 알아보기
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카탈로그 번호수량
K481001
K4810-01으로도 사용됨
20 reactions
카탈로그 번호 K481001
K4810-01으로도 사용됨
제품 가격(KRW)
1,548,000
Each
-
카트에 추가하기
수량:
20 reactions
제품 가격(KRW)
1,548,000
Each
카트에 추가하기
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The GFP Fusion TOPO™ TA Expression Kits are designed to allow fusion of a protein of interest to the Cycle 3 GFP protein. These kits provide topoisomerase-I activated pcDNA3.1/NT-GFP-TOPO™ or pcDNA3.1/CT-GFP-TOPO™ vectors (Figure 1). Taq-amplified DNA fragments are ligated into these vectors in a simple 5-minute reaction right on your bench top. The vectors are designed for high-level expression of transient or stable GFP fusion proteins in a wide range of mammalian cells (Figure 2). Expression is easily detected in living cells using fluorescence. In addition, recombinant proteins expressed from these vectors can be detected on western blots using GFP Antiserum.
For Research Use Only. Not for use in diagnostic procedures.
사양
구성 또는 유도성 시스템Constitutive
배달 유형Transfection
용도(애플리케이션)Reporter Assays
제품 유형TOPO Expression Kit
수량20 reactions
리포터 유전자GFP (Cycle 3)
선택 제제(진핵)Geneticin™ (G-418)
벡터TOPO-TA Vectors
클로닝 방법TOPO-TA
제품라인TOPO™
프로모터CMV
단백질 태그GFP (Cycle 3)
Unit SizeEach
구성 및 보관
GFP Fusion TOPO™ Expression Kits contain two boxes. The GFP Fusion TOPO™ TA Cloning box contains linearized and topoisomerase I-activated cloning vector, dNTPs, salt solution control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a GFP expression control plasmid. Store at -20°C. The One Shot ¤ box contains transformation reagents including single-use, 50-μl aliquots of One Shot™ TOP10 Chemically Competent E. coli, S.O.C. medium, and supercoiled control plasmid. Store the One Shot™ box at -80°C. Kits are guaranteed stable for 6 months when properly stored.
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