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Invitrogen™
pcDNA™3.1 Directional TOPO™ Expression Kit
Catalog number: K490040
Have Questions? 
Invitrogen™
pcDNA™3.1 Directional TOPO™ Expression Kit
Catalog number: K490040
Have Questions? Catalog Number
K490040
Unit Size
40 rxns
Price (USD)
2,041.65 Online price
2,105.00
Availability
-
Quantity
Quantity
| Catalog Number | Unit Size | Price (USD) | Availability | Quantity | |
|---|---|---|---|---|---|
| K490040 | 40 rxns | 2,041.65 Online price 2,105.00 | - | ||
| K490001 | 20 reactions | 1,130.65 Online price 1,172.00 | - |
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The pcDNA™3.1 Directional TOPO™ Expression Kit uses linearized, topoisomerase I-activated pcDNA3.1D/V5-His-TOPO™ for five-minute directional cloning and subsequent high-level expression.
Directional Cloning technology facilitates expression experiments because:
• A proofreading enzyme is used for fewer errors in cloned genes
• Greater than 90% of the clones are in the correct orientation for expression, reducing time spent colony screening for clone orientation
In addition, pcDNA3.1D/V5-His-TOPO™ provides strong expression levels from the CMV promoter and the option of a C-terminal V5-His fusion tag for easy detection of recombinant protein with an Anti-V5 Antibody and rapid purification on nickel-chelating resin.
Directional Cloning technology facilitates expression experiments because:
• A proofreading enzyme is used for fewer errors in cloned genes
• Greater than 90% of the clones are in the correct orientation for expression, reducing time spent colony screening for clone orientation
In addition, pcDNA3.1D/V5-His-TOPO™ provides strong expression levels from the CMV promoter and the option of a C-terminal V5-His fusion tag for easy detection of recombinant protein with an Anti-V5 Antibody and rapid purification on nickel-chelating resin.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Constitutive or Inducible System
Constitutive
Delivery Type
Transfection
Promoter
CMV
Product Type
TOPO Expression Kit
Selection Agent (Eukaryotic)
Geneticin™ (G-418)
Protein Tag
His Tag (6x), V5 Epitope Tag
Cloning Method
Directional TOPO
Quantity
40 Reactions
Vector
Directional TOPO Vectors, pcDNA
Product Line
TOPO™, pcDNA™
For Use With (Application)
Constitutive Expression
Contents & Storage
2 × Box 1 (Store at -20°C):
• 20 μl pcDNA™3.1D/V5-His-TOPO™ (15–20 ng/μl)
• 10 μl dNTP Mix
• 50 μl Salt Solution
• 1 ml Sterile Water
• 20 μl T7 Sequencing Primer (0.1 μg/μl)
• 20 μl BGH Reverse Sequencing Primer (0.1 μg/μl)
• 10 μl Control PCR Template (0.1 μg/μl)
• 10 μl Control PCR Primers (0.1 μg/μl each)
• 10 μl Expression Plasmid (0.5 μg/μl)
2 × Box 2 (Store at -80°C):
• 6 ml S.O.C. Medium (may be stored at 4°C or room temperature)
• 21 × 50 μl TOP10 Cells
• 50 μl pUC19 Control DNA
• 20 μl pcDNA™3.1D/V5-His-TOPO™ (15–20 ng/μl)
• 10 μl dNTP Mix
• 50 μl Salt Solution
• 1 ml Sterile Water
• 20 μl T7 Sequencing Primer (0.1 μg/μl)
• 20 μl BGH Reverse Sequencing Primer (0.1 μg/μl)
• 10 μl Control PCR Template (0.1 μg/μl)
• 10 μl Control PCR Primers (0.1 μg/μl each)
• 10 μl Expression Plasmid (0.5 μg/μl)
2 × Box 2 (Store at -80°C):
• 6 ml S.O.C. Medium (may be stored at 4°C or room temperature)
• 21 × 50 μl TOP10 Cells
• 50 μl pUC19 Control DNA
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