Search Thermo Fisher Scientific
Invitrogen™
ViraPower™ HiPerform™ Lentiviral Gateway™ Expression Kit
Catalog number: K533000
Have Questions? 
Invitrogen™
ViraPower™ HiPerform™ Lentiviral Gateway™ Expression Kit
Catalog number: K533000
Have Questions? Catalog Number
K533000
also known as K5330-00
Unit Size
1 kit
Price (USD)
3,060.00
Availability
-
Quantity
| Catalog Number | Unit Size | Price (USD) | Availability | Quantity | |
|---|---|---|---|---|---|
| K533000 also known as K5330-00 | 1 kit | 3,060.00 | - |
Product Overview
Figures
Videos
Recommendations
Recommendations
Documents
FAQ
Citations & References
Additional Information
Recommendations
The ViraPower™ HiPerform™ Lentiviral Gateway™ Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. This kit combines Invitrogen's™ ViraPower™ HiPerform™ Lentiviral and Gateway™ technologies to facilitate easy recombination-based cloning and lentiviral-based, high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3/V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.
Advantages
- Stable expression
- Long-term experiments
- Accurate titer of functional virus
- Flexible and versatile Gateway™ recombination cloning technology
Key Features
- HiPerform™ WPRE and cPPT elements
- CMV promoter
- V5 epitope tag at C terminus
- Blasticidin selection
Kit includes
- pLenti6.3/V5-DEST™ Gateway™ Vector Kit (V533-06)
- ViraPower™ Bsd Lentiviral Support Kit (K4970-00)
- 293FT Cell Line (R700-07)
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cloning Method
Gateway
Constitutive or Inducible System
Constitutive
Delivery Type
Lentiviral
Cell Type
293-FT
Format
Kit
Promoter
CMV
Vector
pLenti, TOPO-TA Vector
For Use With (Equipment)
HiPerform™, TOPO ™ , ViraPower™
For Use With (Application)
Protein Expression
Key Functions
Viral Expression
Product Line
Gateway™, HiPerform™, ViraPower™
Product Type
Expression Kit
Protein Tag
V5 Epitope Tag
Quantity
1 Kit
Selection Agent (Eukaryotic)
Blasticidin
Special Elements
WPRE, cPPT
Contents & Storage
Vector kit
• pLenti6.3/V5-DEST™ (150 ng/μL), 40 μL, store at -20°C
• pLenti6.3/V5-GW/lacZ Control vector (0.5 μg/μL), 20 μL, store at -20°C
• One Shot™ Stbl3™ Chemically Competent E. coli, 21 x 50 μL, store at -80°C
• pUC19 control DNA (10 pg/μL), 50 μL, store at -80°C
• S.O.C. Medium, 6 mL, store at -80°C
Support kit
• ViraPower™ Packaging Mix, 195 μg, store at -20°C
• Lipofectamine™ 2000, 0.75 mL, store at 4°C
• Blasticidin, 50 mg, store at -20°C
• 293 FT cells (3 x 106 cells/mL), 1 mL, store in liquid nitrogen
Figures
Documents & Downloads
Certificates
Search by lot number or partial lot number
Frequently asked questions (FAQs)
Citations & References
Search citations by name, author, journal title or abstract text