IMAGEN™ Parainfluenza Virus Typing Kit using Direct Immunofluorescence Assay

Rapidly detect and differentiate Parainfluenza virus types 1, 2 and 3 antigens directly in nasopharyngeal aspirates or in cell culture preparations with Thermo Scientific™ IMAGEN™ Parainfluenza Virus Typing Kit. This direct immunofluorescence assay utilizes type-specific monoclonal antibodies to detect and identify Parainfluenza virus types 1, 2 and 3

Parainfluenza viruses are members of the genus Paramyxovirus classified within the family Paramyxoviridae.¹ There are 6 species of Paramyxovirus known to infect humans which include Parainfluenza viruses 1, 2, 3, and 4 (subtypes 4a and 4b), Mumps virus, and Newcastle Disease virus. Of the 4 Parainfluenza viruses, types 1, 2, and 3 are now recognized as a major cause of acute respiratory disease in infants and children.^2,3

Use IMAGEN Parainfluenza Kit as a rapid, sensitive, and specific method for detection and differentiation of Parainfluenza virus types 1, 2, and 3 antigens directly in nasopharyngeal aspirates or in cell culture preparations.

• Rapid results—within a little over 30 minutes of receiving test sample or culture
• Simple—ready-to-use reagents plus all IMAGEN assays follow the same basic methodology
• Excellent sensitivity and specificity—high quality, well-characterized antibodies used in the assay provide excellent sensitivity and specificity
• Easy-to-read results
• Long shelf life—24 months

The IMAGEN Parainfluenza Kit contains fluorescein isothiocyanate (FITC) conjugated monoclonal antibodies which bind specifically to either Parainfluenza virus types 1, 2 or 3. Specimens are incubated with the FITC conjugated reagents for 15 minutes. Excess reagent is then removed by washing with phosphate-buffered saline (PBS). The stained areas are mounted and viewed using epifluorescence illumination. If Parainfluenza virus types 1, 2, or 3 are present then characteristic apple-green intracellular fluorescence is seen within infected cells, which contrasts with the red background staining of uninfected cells.

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