MagicMedia™ E. coli Expression Medium, Powder
MagicMedia&trade; <i>E. coli</i> Expression Medium, Powder
Invitrogen™

MagicMedia™ E. coli Expression Medium, Powder

MagicMedia E. coli Expression Medium is specifically designed to dramatically increase the yield of recombinant proteins in T7-regulated E. coliRead more
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Catalog NumberQuantity
K68155 x 1 L
K68101 L
Catalog number K6815
Price (USD)
412.65
Online Exclusive
441.00
Save 28.35 (6%)
Each
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Quantity:
5 x 1 L
Price (USD)
412.65
Online Exclusive
441.00
Save 28.35 (6%)
Each
Add to cart
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MagicMedia E. coli Expression Medium is specifically designed to dramatically increase the yield of recombinant proteins in T7-regulated E. coli expression systems without monitoring culture OD (optical density) or adding inducer. The proprietary MagicMedia formulation enables E. coli growth to reach culture densities 3–10-fold higher than those achieved with traditional LB + IPTG methods, allowing you to:

• Achieve 3–10 times higher protein yields (Figure 1) with much less hands-on time
• Eliminate time-consuming OD monitoring steps—simply inoculate, grow the culture overnight, and harvest (Figure 2)
• Reduce culture volume to simplify handling and scale-up processes
• Ideal for simultaneous expression of multiple clones for high-throughput applications

MagicMedia E. coli Expression Medium, powder, consists of a proprietary Component A (in foil pouch powder format) and Component B (in buffer liquid format).

MagicMedia E. coli Expression Medium is also available in a ready-to-use, pre-sterilized liquid format (Cat. No. K6803).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormatKit
Media TypeE. coli Expression Media
Preparation MethodAutoclave
Product LineMagicMedia™
Quantity5 x 1 L
FormPowder
Product TypeExpression Medium
SpeciesEscherichia coli
Unit SizeEach
Contents & Storage
Component A (5 x 1 foil pouches) and Component B buffer (5 x 1 bottle liquid).

Store at room temperature (15–30°C).
All components guaranteed stable for six months when stored properly.

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Frequently asked questions (FAQs)

Can I use a lower incubation temperature for MagicMedia Medium?

Yes, expression at 18 degrees C and 25 degrees C has been validated using the Dual Temperature expression protocol.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

I am using a Champion pET vector with the BL21-AI cells. Can I use your MagicMedia Medium for expression?

Unfortunately, MagicMedia Medium will not work with the arabinose inducer. MagicMedia Medium needs an IPTG-inducible system along with an intact Lac operon.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is MagicMedia Medium?

MagicMedia Medium is an E. coli expression medium. It produces 3-10 times higher protein yield, is compatible with T7 regulated E. coli vectors that contain a functional lac operon and BL21 (DE3) strains, is simple to use, and eliminates OD monitoring/induction steps.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

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