DreamTaq™ Hot Start PCR Master Mixes
DreamTaq™ Hot Start PCR Master Mixes
Thermo Scientific™

DreamTaq™ Hot Start PCR Master Mixes

Thermo Scientific DreamTaq Hot Start PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq Hot Start DNA Polymerase, DreamTaq buffer, magnesium, and dNTPs.
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Catalog NumberColorNo. of Reactions
K9021Green200 Reactions
K9011Colorless200 Reactions
K9012Colorless1000 Reactions
K9022Green1000 Reactions
Catalog number K9021
Price (USD)
189.65
Online Exclusive
214.00
Save 24.35 (11%)
Each
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Color:
Green
No. of Reactions:
200 Reactions
Request bulk or custom format
Price (USD)
189.65
Online Exclusive
214.00
Save 24.35 (11%)
Each
Add to cart
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Thermo Scientific DreamTaq Hot Start PCR Master Mix, available in colorless and green formats, is a ready-to-use 2X reaction mix that includes DreamTaq Hot Start DNA Polymerase, DreamTaq Buffer, magnesium, and dNTPs.

The master mix allows easy reaction setup and fewer pipetting steps, with only primers, template, and water needing to be added. The DreamTaq Hot Start Green PCR Master Mix includes a density reagent and two tracking dyes for convenient direct loading of PCR products on a gel.

DreamTaq Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases. DreamTaq Hot Start DNA Polymerase employs antibody-based inhibition of DNA polymerase activity at ambient temperatures to prevent the amplification of non-specific products prior to the amplification step. With DreamTaq Hot Start DNA Polymerase, reactions can be set up at room temperature using the same protocol and cycling conditions as conventional Taq DNA polymerases.

DreamTaq Hot Start PCR Master Mix is formulated to enhance productivity through:

  • Better reaction outcomes
    • Higher yield of target amplicons from low template amounts
    • Increased reaction specificity due to reliable hot-start technology
    • Wider range of amplicon lengths with routine amplification of genomic DNA fragments up to 6 kb
  • Enhanced convenience
    • Room temperature reaction setup
    • Minimized optimization of Mg2+ concentration and of primer annealing temperatures due to optimized reaction buffer
    • Simplified workflow and minimized pipetting steps with 2X master mix format
    • Direct loading of PCR products on a gel with green format

Applications

  • Generation of PCR products for TA cloning
  • Routine PCR
  • Colony PCR
  • Genotyping
  • RT-PCR

For Research Use Only. Not for use in diagnostic procedures.

Specifications
ColorGreen
Concentration2X
Hot StartBuilt-In Hot Start
No. of Reactions200 Reactions
Overhang3'-A
PolymeraseDreamTaq DNA Polymerase
Quantity200 rxns
Reaction FormatSuperMix or Master Mix
Shipping ConditionDry Ice
For Use With (Application)Hot-start PCR
GC-Rich PCR PerformanceLow
Reaction SpeedStandard
Unit SizeEach
Contents & Storage
• 2X DreamTaq Hot Start Green PCR Master Mix, 4 x 1.25 mL
• Nuclease-free water, 4 x 1.25 mL

Store at -5°C to -30°C.
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Frequently asked questions (FAQs)

Which nucleotide analogues can be used with DreamTaq Hot Start DNA Polymerase?

DreamTaq Hot Start DNA Polymerase can incorporate dUTP and a variety of modified nucleotides, such as: dITP, 7-deaza-dGTP, fluorescein-12-dUTP, Biotin-11-dUTP, dm5CTP, alpha-thio-dCTP, and aminoallyl-dUTP.

Are DreamTaq buffers the same for DreamTaq DNA Polymerase and DreamTaq Hot Start DNA Polymerase?

Yes. 10X DreamTaq Buffer and 10X DreamTaq Green Buffer are the same for both polymerases.

My experiments require extra DreamTaq Buffer. Do you offer the buffer as a separate product?

Yes, DreamTaq Buffer (Cat. No. B65, 4 x 1.25 mL) and DreamTaq Green Buffer (Cat. No. B71, 4 x 1.25 mL) are available as stand-alone products.

Can DreamTaq Hot Start DNA Polymerase be used for amplification of bisulfite converted DNA?

Yes. DreamTaq Hot Start DNA Polymerase can read uracil in the template strand and therefore can be used for amplification of bisulfite converted DNA.

What is the limit of amplicon size using DreamTaq Hot Start DNA Polymerase?

DreamTaq Hot Start DNA Polymerase amplifies up to 6 kb from human genomic DNA and up to 20 kb from lambda DNA with high yields and specificity. Amplification of even longer fragments up to 9 kb from human genomic DNA has been demonstrated, but may require additional optimization of reaction conditions and primer design.

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