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PicoPure™ RNA Isolation Kit
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Applied Biosystems™

PicoPure™ RNA Isolation Kit

The Arcturus™ PicoPure™ RNA Isolation Kit is designed to recover high-quality total RNA consistently from fewer than ten cells, evenRead more
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Catalog NumberNo. of Samples
KIT020440 samples
KIT0214200 samples
Catalog number KIT0204
Price (USD)
606.00
Each
In stock
Add to cart
No. of Samples:
40 samples
Price (USD)
606.00
Each
Add to cart
The Arcturus™ PicoPure™ RNA Isolation Kit is designed to recover high-quality total RNA consistently from fewer than ten cells, even from a single cell. This kit is designed to provide consistent, efficient RNA recovery without sacrificing quality. Small volume elution allows you to maximize your recovery of RNA from small numbers of cells for use with your gene expression analysis.

Key Product Features:

• Greater sensitivity—isolate RNA from even a single cell
• Superior efficiency—maximize RNA recovery from samples
• Streamlined concentration—elute with small volumes
• Flexible application—compatible with many tube sizes
• Integrated contamination prevention—column lids ensure that samples remain RNase free

Isolate RNA from Even a Single Cell
The PicoPure™ RNA Isolation Kit is engineered to recover high-quality total RNA consistently from fewer than ten cells. The high recovery rate attained using this kit is valuable not only for small samples containing picograms of RNA but also for samples containing up to 100 μg of RNA. Since the RNA elutes in as little as 10 μl of buffer, it is ready for gene expression analysis without any vacuum concentration. This kit is available in two sizes, enough for 40 or 200 isolations.

Get Superior RNA Recovery
Small, valuable samples often cannot be replaced. Performing gene expression studies on these precious samples requires getting maximum RNA recovery. Comparative studies show that, with small, RNA-poor cell or tissue samples, the PicoPure™ Kit dramatically outperforms other commercial total RNA isolation kits in efficiency.

Eliminate Time-consuming RNA Concentration
The simple isolation protocol consists of extracting cellular RNA, then loading the extract onto the specially designed MiraCol™ Purification Column to bind the RNA. After washing away impurities, the RNA elutes in only 10 μl of buffer, ready for use without vacuum concentration or transfer into a new reaction tube.

High Yield Purification Columns
The PicoPure™ RNA Isolation Kit features unique, high-recovery MiraCol™ Purification Columns, engineered to maximize recovery from microscopic samples. The columns have convenient integrated lids to help ensure that samples remain RNase free. In addition, the Kit comes complete with all necessary buffers, an easy-to-follow User Guide, optimized protocols for handling Laser Capture Microdissected (LCM) samples, and protocols for isolating RNA from larger samples.

Deliver RNA Ready for Amplification and Microarray Analysis
RNA isolated with the PicoPure™ RNA Kit is ready, without vacuum concentration, for amplification with the RiboAmp™ RNA Amplification Kit. The two kits together allow for highly reproducible gene expression results.

Retain Low-abundance mRNA
The PicoPure™ RNA Isolation Kit preserves mRNA in all abundance classes through the isolation process, minimizing the loss of important genes of interest. Low, medium, and high-abundance genes are consistently recovered and available for expression analysis.

Maintain High RNA Quality
The PicoPure™ RNA isolation process allows for high-quality RNA. Total cellular RNA prepared from LCM samples remains intact after isolation, increasing confidence in subsequent molecular analysis.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Final Product TypeTotal RNA
For Use With (Application)Microarray Analysis, Real-Time Quantitative PCR (qPCR)
For Use With (Equipment)ArcturusXT™ LCM Instrument
High-throughput CompatibilityNot High-throughput Compatible (Manual)
No. of Samples40 samples
Product LinePicoPure™
Quantity40 samples
Starting Material AmountAs little as a single cell.
FormatSpin Column
Isolation TechnologySpin Column
Sample TypeLaser Capture Microdissection (LCM), Tissue (Frozen)
TargetTotal RNA
Unit SizeEach
Contents & Storage
Extraction buffer, purification reagents, purification columns, microcentrifuge tubes. Store at room temperature

Frequently asked questions (FAQs)

How many cells are needed for the PicoPure RNA Isolation Kit?

The PicoPure RNA Isolation Kit can be used with as little as a single cell to samples with up to 100 µg of RNA.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What are the RNA size limits for PicoPure RNA Isolation Kit?

The PicoPure RNA Isolation Kit is not recommended for isolation of RNA smaller than 100 nucleotides.

I am having trouble assessing the RNA quality from formalin-fixed, paraffin-embedded samples after laser capture microdissection (LCM). Can you offer some tips?

Formalin-fixed paraffin-embedded (FFPE) samples introduce unique challenges for gene expression profiling and gene expression quantitation, including chemical modification and fragmentation of RNA molecules. The initial paraffin blot preparation and storage significant affect the RNA quality. Archival FFPE samples present additional challenges due to increased RNA degradation over time. As a result, not all FFPE samples contain high quality RNA. 

In addition, a typical Bioanalyzer profile of RNA from an FFPE sample can look fragmented and degraded and as a result, that approach may not be useful in determining the transcribability of your RNA. Therefore, a tissue scrape test is recommended. You can use the entire adjacent section to isolate RNA and to access the RNA quantity and quality for intact RNA. Two sets of β-actin gene-specific primers designed to amplify short segments of DNA within the β-actin target sequence from the 5’ and 3’ of the β-actin gene can be used to estimate the amount of RNA in a given sample in relation to β-actin content using real-time PCR. The RNA quantity derived from the 3' primer set is used to quantitate the RNA in the FFPE tissue scrape. The ratio of the RNA yield obtained from both sets of PCR primers is the 3'⁄5' ratio and is used as an indication of RNA quality. If there is less product yield of the 5’ β-actin primers, it indicates that there is less full length of RNA.

I am getting poor quality of RNA from my LCM samples. How can I improve it?

The dissection and embedding steps are critical for the RNA quality for LCM samples. We suggest using the entire adjacent section or dissecting a large area of the section (>1000 cells) for RNA isolation. Before proceeding to LCM microdissection, we recommend assessing the quality of RNA isolated from the adjacent section. With good amount of RNA, one can assess the RNA quantity and quality using the Qubit RNA HS Assay Kit and the Agilent Bioanalyzer. This will help to make sure that good quality RNA is obtained from a low number of LCM cells.

What samples can be used with the Arcuturus PicoPure RNA Isolation Kit?

The Arcturus PicoPure RNA Isolation Kit removes total cellular RNA from pico-scale samples including frozen sections, alcohol fixed-, or fresh samples. Please note, samples fixed with formaldehyde are not recommended for use with this kit.

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