Thermo Scientific Chemicals

Fast Blue BB salt

Catalog number: L09704.06
5 g, Each
Thermo Scientific Chemicals

Fast Blue BB salt

Catalog number: L09704.06
5 g, Each
Quantity
Catalog number: L09704.06
also known as L09704-06
Price (USD)
Quantity
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Chemical Identifiers

CAS
5486-84-0
IUPAC Name
4-benzamido-2,5-diethoxybenzene-1-diazonium
Molecular Formula
C17H18N3O3
InChI Key
DKPBKNZQVUOHRQ-UHFFFAOYSA-O
SMILES
CCOC1=CC([N+]#N)=C(OCC)C=C1NC(=O)C1=CC=CC=C1
Form
powder
Assay (unspecified)
> 80.0% dye content
Appearance (Color)
Yellow to green or dark brown

Description

Fast Blue BB salt is used as a general substrate for β-glycosidase. Two moles of 6-bromo-2-napthol-β-d-glucopyranoside are cleaved by β-glucosidases into 2 moles of d-glucose and 2 moles of 6-bromo-2-napthol. The 2 moles of 6-bromo-2-napthol formed are coupled with 1 mole of Fast Blue BB salt [4-benzoylamino-2,5-diethoxybenzenediazonium chloride hemi (zinc chloride) salt], resulting in 2 moles of the corresponding dye of 6-bromo-2-naphthol (reddish color). It is a commonly used stain to observe myelin under light microscopy.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
Fast Blue BB salt is used as a general substrate for β-glycosidase. Two moles of 6-bromo-2-napthol-β-d-glucopyranoside are cleaved by β-glucosidases into 2 moles of d-glucose and 2 moles of 6-bromo-2-napthol. The 2 moles of 6-bromo-2-napthol formed are coupled with 1 mole of Fast Blue BB salt [4-benzoylamino-2,5-diethoxybenzenediazonium chloride hemi (zinc chloride) salt], resulting in 2 moles of the corresponding dye of 6-bromo-2-naphthol (reddish color). It is a commonly used stain to observe myelin under light microscopy.

Solubility
Soluble in water.
RUO – Research Use Only

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