Search Thermo Fisher Scientific
Invitrogen™
LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells
The LIVE/DEAD® Viability/Cytotoxicity Kit is a quick and easy two-color assay to determine viability of cells in a population basedRead more
| Catalog Number | Quantity |
|---|---|
| L3224 | 1 kit |
Catalog number L3224
Price (USD)
696.00
Each
-
Quantity:
1 kit
Price (USD)
696.00
Each
The LIVE/DEAD® Viability/Cytotoxicity Kit is a quick and easy two-color assay to determine viability of cells in a population based on plasma membrane integrity and esterase activity. The kit can be used in flow cytometry, fluorescence microscopy, and with fluorescence microplate readers.
Advantages Over Alternative Methods Include:
• Faster
• Safer
• More sensitive
• Less expensive
Easily Use with a Wide Variety of Techniques and Cell Types
Ubiquitous intracellular esterase activity and an intact plasma membrane are distinguishing characteristics of live cells. The LIVE/DEAD® Viability/Cytotoxicity Kit quickly discriminates live from dead cells by simultaneously staining with green-fluorescent calcein-AM to indicate intracellular esterase activity and red-fluorescent ethidium homodimer-1 to indicate loss of plasma membrane integrity. It is adaptable to most eukaryotic cells where cytotoxic conditions produce these cellular effects. The assay is useful with a variety of fluorescence-detection methodologies.
Sensitive, Safe, and Efficient
The LIVE/DEAD® Viability/Cytotoxicity Kit is more sensitive than Trypan blue exclusion, a commonly used method for live/dead cell discrimination. The cost-effective LIVE/DEAD® Viability/Cytotoxicity Kit is highly sensitive due to the bright fluorescence of both dyes upon interacting with either live (for calcein-AM) or dead (for ethidium homodimer-1) cells. Background levels are low due to the fact that both dyes are virtually non-fluorescent prior to interacting with cells.
LIVE/DEAD® Assays Available for a Broad Range of Applications
A selection of Invitrogen LIVE/DEAD® Viability Assays is offered for mammalian cells, bacteria, yeast and fungi, as well as Fixable Dead Cell Stain Kits for use in intracellular staining for flow cytometry. All LIVE/DEAD® assays provide quick, positive discrimination between viable and non-viable cells.
Related Link
• Learn More and See the Full Range of LIVE/DEAD® Assay Products.
Advantages Over Alternative Methods Include:
• Faster
• Safer
• More sensitive
• Less expensive
Easily Use with a Wide Variety of Techniques and Cell Types
Ubiquitous intracellular esterase activity and an intact plasma membrane are distinguishing characteristics of live cells. The LIVE/DEAD® Viability/Cytotoxicity Kit quickly discriminates live from dead cells by simultaneously staining with green-fluorescent calcein-AM to indicate intracellular esterase activity and red-fluorescent ethidium homodimer-1 to indicate loss of plasma membrane integrity. It is adaptable to most eukaryotic cells where cytotoxic conditions produce these cellular effects. The assay is useful with a variety of fluorescence-detection methodologies.
Sensitive, Safe, and Efficient
The LIVE/DEAD® Viability/Cytotoxicity Kit is more sensitive than Trypan blue exclusion, a commonly used method for live/dead cell discrimination. The cost-effective LIVE/DEAD® Viability/Cytotoxicity Kit is highly sensitive due to the bright fluorescence of both dyes upon interacting with either live (for calcein-AM) or dead (for ethidium homodimer-1) cells. Background levels are low due to the fact that both dyes are virtually non-fluorescent prior to interacting with cells.
LIVE/DEAD® Assays Available for a Broad Range of Applications
A selection of Invitrogen LIVE/DEAD® Viability Assays is offered for mammalian cells, bacteria, yeast and fungi, as well as Fixable Dead Cell Stain Kits for use in intracellular staining for flow cytometry. All LIVE/DEAD® assays provide quick, positive discrimination between viable and non-viable cells.
Related Link
• Learn More and See the Full Range of LIVE/DEAD® Assay Products.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeMammalian Cells
DescriptionLIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells
Detection MethodFluorescence
Dye TypeOther Label(s) or Dye(s)
FormatTube(s), 96-well plate, Slide(s)
Quantity1 kit
Shipping ConditionRoom Temperature
ColorGreen, Red
Emission517/617
Excitation Wavelength Range494, 528 nm
For Use With (Application)Viability Assay
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer, Microplate Reader
Product LineLIVE/DEAD
Product TypeViability/Cytotoxicity Kit
Unit SizeEach
Contents & Storage
Store in freezer at -5°C to -30°C and protect from light.
Have questions about this product? Ask our AI assisted search.
This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our
Privacy Notice.
