LysoSensor™ Green DND-189 - Special Packaging
LysoSensor™ Green DND-189 - Special Packaging
Invitrogen™

LysoSensor™ Green DND-189 - Special Packaging

LysoSensor Green DND-189 can be used to measure the pH of acidic organelles, such as lysosomes. The LysoSensor dye becomesRead more
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Catalog NumberQuantity
L753520 x 50 μL
Catalog number L7535
Price (USD)
518.00
Each
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Quantity:
20 x 50 μL
Price (USD)
518.00
Each
Add to cart
LysoSensor Green DND-189 can be used to measure the pH of acidic organelles, such as lysosomes. The LysoSensor dye becomes more fluorescent in acidic environments. The pKa of LysoSensor Green DND-189 is ∼5.2.

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For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration1 mM
Detection MethodFluorescence
Quantity20 x 50 μL
Shipping ConditionRoom Temperature
Sub Cellular LocalizationLysosomes
ColorGreen
Emission443/505
For Use With (Equipment)Fluorescence Microscope
Product LineLysoSensor
Product TypeDye
Unit SizeEach
Contents & Storage
Store in freezer -5°C to -30°C and protect from light.
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Frequently asked questions (FAQs)

What is the working concentration for LysoSensor Green DND-189 (Cat. No. L7535)?

We recommend diluting the 1 mM stock solution to a working concentration of at least 1 µM using the growth medium or buffer of choice. To reduce potential artifacts from overloading, the concentration of dye should be kept as low as possible.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use a plate reader to measure the pH of acidic organelles, such as lysosomes, with LysoSensor ratiometric probes?

Using a plate reader for measuring the pH of acidic organelles, such as lysosomes, with LysoSensor ratiometric probes, provides weak pH measurements, as discrete regions of interest cannot be selected for analysis. Therefore, there is no way of excluding extracellular fluorescence and little to no spatial or morphological information can be obtained. Instead, a readout of whole-cell fluorescence is provided, resulting in the average intraorganellar pH of all labeled organelles for the analyzed cell population. The is also the risk that a signal may be dominated by a few extremely bright, but essentially irrelevant cells, such as dead cells, or dye-labeled debris particles.

For more information about measuring luminal pH in organelles, please see the reference linked below:

Live-cell Microscopy and Fluorescence-based Measurement of Luminal pH in Intracellular Organelles

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What reagents would you recommend for measuring the pH in acidic organelles, such as lysosomes?

For measuring the pH in acidic organelles, such as lysosomes, we recommend our LysoSensor ratio metric probes. Please see the links to the product page and descriptions below:

LysoSensor Yellow/Blue dextran, 10,000 MW, Anionic, Fixable (Cat. No. L22460):
LysoSensor Yellow/Blue dextran is a ratiometric probe that can be used to measure the pH of acidic organelles, such as lysosomes. This dextran conjugate is taken up by the cells through endocytosis. The LysoSensor dye produces blue fluorescence in neutral environments, but changes to yellow fluorescence in more acidic environments. The pKa of LysoSensor Yellow/Blue dextran is ~3.9.

LysoSensor Yellow/Blue DND-160 - Special Packaging (Cat. No. L7545):
LysoSensor Yellow/Blue DND-160 is a ratiometric probe that can be used to measure the pH of acidic organelles, such as lysosomes. The LysoSensor dye produces blue fluorescence in neutral environments, but changes to yellow fluorescence in more acidic environments. The pKa of LysoSensor Yellow/Blue DND-160 is ~4.2.

LysoSensor Blue DND-167 - Special Packaging (Cat. No. L7533):
LysoSensor Blue DND-167 can be used to measure the pH of acidic organelles, such as lysosomes. The LysoSensor dye becomes more fluorescent in acidic environments. The pKa of LysoSensor Blue DND-167 is ~5.1.

LysoSensor Green DND-189 - Special Packaging (Cat. No. L7535):
LysoSensor Green DND-189 can be used to measure the pH of acidic organelles, such as lysosomes. The LysoSensor dye becomes more fluorescent in acidic environments. The pKa of LysoSensor Green DND-189 is ~5.2.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

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Fluorescence spectra

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