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            • Primary Antibodies ›
            • HLA-G Antibodies

            Invitrogen

            HLA-G (soluble) Monoclonal Antibody (2A12)

            View all (58) HLA-G antibodies
            Datasheet
            Protocols
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            Datasheet
            Protocols
            Questions & Answers

            Cite HLA-G (soluble) Monoclonal Antibody (2A12)

            • Antibody Testing Data (2)
            Application
            HLA-G (soluble) Antibody in Flow Cytometry (Flow)
            Group 53 Created with Sketch.

            FIGURE: 1 / 2

            HLA-G (soluble) Antibody (MA1-10358) in Flow

            Separation of HLA-G transfected HEK-293 cells (red-filled) from HLA-G negative debris (black-dashed) in flow cytometry analysis (intracellular staining) of HLA-G transfected HEK-293 cells using anti-human HLA-G (2A12) purified Monoclonal antibody (Product # MA1-10358) (concentration in sample 4 µg/mL) GAM APC.
            View Product

            HLA-G (soluble) Antibody in Flow Cytometry (Flow)
            HLA-G (soluble) Antibody in Flow Cytometry (Flow)

            Product Details

            MA1-10358

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            Assay-dependent
            -

            Immunohistochemistry (Paraffin) (IHC (P))

            Assay-dependent
            -

            Immunohistochemistry (Frozen) (IHC (F))

            Assay-dependent
            -

            Flow Cytometry (Flow)

            1-4 µg/mL
            -

            ELISA (ELISA)

            Assay-dependent
            -
            Product Specifications

            Species Reactivity

            Human

            Host/Isotype

            Mouse / IgG1

            Class

            Monoclonal

            Type

            Antibody

            Clone

            2A12

            Immunogen

            C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupled to ovalbumin.
            View immunogen

            Conjugate

            Unconjugated
            • FITC
            • Request custom conjugation

            Form

            Liquid

            Concentration

            1 mg/mL

            Purification

            sequential chromatography

            Storage buffer

            PBS, pH 7.4

            Contains

            15mM sodium azide

            Storage conditions

            4°C, do not freeze

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_11156617

            Product Specific Information

            This antibody was generated to a peptide corresponding to C-intron 4-encoded sequence. This antibody does not cross-react with the full-length HLA-G1 isoform and thus allows to distinguish between secreted HLA-G5 and HLA-G6 isoforms from shedded HLA-G1.

            Target Information

            HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail.

            HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            References (0)

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            Cite this product

            Bioinformatics

            Protein Aliases: b2 microglobulin; DADB-15K14.8; HLA class I histocompatibility antigen, alpha chain G; HLA class I molecule; HLA G antigen; HLA-G histocompatibility antigen, class I, G; MHC class I antigen G

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            Gene Aliases: HLA-6.0; HLA-G; HLAG; MHC-G

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            UniProt ID: (Human) P17693

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            Entrez Gene ID: (Human) 3135

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            Function(s)

            antigen binding receptor binding peptide antigen binding protein homodimerization activity major histocompatibility complex protein

            Process(es)

            antigen processing and presentation of peptide antigen via MHC class I antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent positive regulation of tolerance induction positive regulation of T cell tolerance induction immune response-inhibiting cell surface receptor signaling pathway cellular defense response antigen processing and presentation positive regulation of interleukin-12 production negative regulation of T cell proliferation positive regulation of regulatory T cell differentiation regulation of immune response negative regulation of immune response interferon-gamma-mediated signaling pathway type I interferon signaling pathway negative regulation of dendritic cell differentiation
            It has to be done as per old AB suggested Products section.
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            If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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