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            • Primary Antibodies ›
            • PCNA Antibodies

            Invitrogen

            PCNA Monoclonal Antibody (PC10), Biotin

            Advanced Verification
            1 Published Figure
            137 References
            View all (154) PCNA antibodies
            Datasheet
            Protocols
            Questions & Answers
            Catalog # MA5-11355
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            Price (USD)
            412.65
            Online Exclusive
            550.00
            Save 137.35 (25%)
            500 µL
             
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            Datasheet
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            Questions & Answers

            Cite PCNA Monoclonal Antibody (PC10), Biotin

            • Antibody Testing Data (7)
            • Published Figures (1)
            • Advanced Verification (1)
            Application
            PCNA Antibody in Western Blot (WB)
            Group 53 Created with Sketch.

            FIGURE: 1 / 9

            PCNA Antibody (MA5-11355) in WB

            Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), MCF7 (Lane 2), Jurkat (Lane 3), HEK-293 (Lane 4), NIH/3T3 (Lane 5), MDA-MB-231 (Lane 6), A549 (Lane 7), SH-SY5Y (Lane 8) and Raji (Lane 9). The blot was probed with anti-PCNA Monoclonal Antibody (Product # MA5-11355, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 34 kDa band corresponding to PCNA was ... View More
            View Product

            PCNA Antibody in Western Blot (WB)
            PCNA Antibody in Western Blot (WB)
            PCNA Antibody in Western Blot (WB)
            PCNA Antibody in Immunocytochemistry (ICC/IF)
            PCNA Antibody in Immunocytochemistry (ICC/IF)
            PCNA Antibody in Flow Cytometry (Flow)
            PCNA Antibody in Immunoprecipitation (IP)
            PCNA Antibody in Immunohistochemistry (IHC)
            PCNA Antibody

            Product Details

            MA5-11355

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:500
            View 29 publications 29 publications

            Immunohistochemistry (IHC)

            -
            View 96 publications 96 publications

            Immunohistochemistry (Paraffin) (IHC (P))

            1:200-1:400
            View 1 publication 1 publication

            Immunocytochemistry (ICC/IF)

            1:20-1:50
            View 8 publications 8 publications

            Flow Cytometry (Flow)

            1:200-1:1,000
            -

            Immunoprecipitation (IP)

            2 µg/mL
            View 2 publications 2 publications

            Miscellaneous PubMed (Misc)

            -
            View 1 publication 1 publication
            Product Specifications

            Species Reactivity

            Human, Rat

            Published species

            Amphibian, Cat, Dog, Guinea pig, Human, Mouse, Non-human primate, Pig, Rabbit, Rat, Rhesus monkey, Xenopus, Yeast, Zebrafish

            Host/Isotype

            Mouse / IgG2a, kappa

            Class

            Monoclonal

            Type

            Antibody

            Clone

            PC10

            Immunogen

            Recombinant rat PCNA protein

            Conjugate

            Biotin
            • Unconjugated
            • Request custom conjugation

            Form

            Liquid

            Concentration

            200 µg/mL

            Purification

            Protein A

            Storage buffer

            PBS, pH 7.4, with 0.2% BSA

            Contains

            0.09% sodium azide

            Storage conditions

            4°C

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_10984046

            Product Specific Information

            Methanol fixation is required for immunofluorescence and flow cytometry staining. To decrease nonspecific staining for both applications, using a Biotin/Streptavidin blocker is recommended.

            By WB, MA5-11355 detects a predominant band between 30-35kD, and fainter nonspecific bands at ~72kD and 130kD.

            Target Information

            PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            Related Products for

            Bioinformatics

            Protein Aliases: Cyclin; DNA polymerase delta auxiliary protein; HGCN8729; MGC8367; PCNA; Proliferating cell nuclear antigen

            View more View less

            Gene Aliases: ATLD2; PCNA; Pcna/cyclin; PCNAR

            View more View less

            UniProt ID: (Human) P12004, (Rat) P04961

            View more View less

            Entrez Gene ID: (Human) 5111, (Rat) 25737

            View more View less

            Function(s)

            purine-specific mismatch base pair DNA N-glycosylase activity chromatin binding damaged DNA binding protein binding enzyme binding estrogen receptor binding DNA polymerase processivity factor activity receptor tyrosine kinase binding dinucleotide insertion or deletion binding MutLalpha complex binding histone acetyltransferase binding identical protein binding DNA polymerase binding transcription factor binding DNA polymerase processivity factor

            Process(es)

            G1/S transition of mitotic cell cycle regulation of transcription involved in G1/S transition of mitotic cell cycle telomere maintenance via recombination telomere maintenance DNA strand elongation involved in DNA replication leading strand elongation transcription-coupled nucleotide-excision repair nucleotide-excision repair, DNA incision, 5'-to lesion nucleotide-excision repair, DNA gap filling mismatch repair DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest heart development cell proliferation protein sumoylation translesion synthesis epithelial cell differentiation replication fork processing positive regulation of deoxyribonuclease activity nucleotide-excision repair, DNA incision response to lipid cellular response to UV error-prone translesion synthesis DNA damage response, detection of DNA damage positive regulation of DNA repair positive regulation of DNA replication response to cadmium ion error-free translesion synthesis mitotic telomere maintenance via semi-conservative replication negative regulation of transcription from RNA polymerase II promoter regulation of DNA replication base-excision repair, gap-filling cellular response to xenobiotic stimulus
            It has to be done as per old AB suggested Products section.
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