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Invitrogen™
NeuroTrace™ 530/615 Red Fluorescent Nissl Stain - Solution in DMSO
Nissl staining is a standard histological method for visualizing neurons in the brain and spinal cord. Composed of ribosomal RNARead more
| Catalog Number | Quantity |
|---|---|
| N21482 | 1 mL |
Catalog number N21482
Price (EUR)
423,00
Each
-
Quantity:
1 mL
Price (EUR)
423,00
Each
Nissl staining is a standard histological method for visualizing neurons in the brain and spinal cord. Composed of ribosomal RNA associated with the rough endoplasmic reticulum in neuronal perikarya and dendrites, the Nissl substance redistributes within the cell body in injured or regenerating neurons, providing a marker for the physiological state of the neuron. Our NeuroTrace 530/615 red fluorescent Nissl stain is selective for the Nissl substance characteristic of neurons and provides more sensitivity than traditional histological dyes like toluidine blue or cresyl violet.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorOrange
Detection MethodFluorescence
For Use With (Equipment)Fluorescence Microscope
Product TypeNissl Stain
Quantity1 mL
Shipping ConditionRoom Temperature
Sub Cellular LocalizationNissl Bodies
Excitation/Emission530/615 nm
Product LineNEUROTRACE™
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -30°C) and protect from light.
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Figures
Mouse Anti-Alpha Tubulin Monoclonal Antibody (Cat. No. A11126)
Mouse cerebellum section. NeuroTrace® 530/615 red-fluorescent Nissl stain and nuclear yellow.
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3050017Certificate of AnalysisOct 28, 2024N21482
2700982Certificate of AnalysisDec 31, 2023N21482
2491416Certificate of AnalysisJul 14, 2022N21482
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Frequently asked questions (FAQs)
Yes. We have done this successfully with an anti-GFAP primary and an Alexa Fluor secondary antibody. We would recommend labeling with the primary and secondary antibodies first, then following up with the standard NeuroTrace Nissl Stain protocol.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Our NeuroTrace Nissl stains label the Nissl substance which is composed of ribosomal RNA associated with the rough endoplasmic reticulum and is present in high amounts in neuronal cells. These dyes are not completely specific for neurons, but will selectively stain neurons based on their high level of protein synthesis. In some cases they may show staining of other cell types such as glia, so you may need to decrease the staining concentration to obtain more selective neuronal labeling. We suggest dilutions in the range of 20- to 300-fold for neuronal staining.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
We have only tested them on mouse brain cryosections, however, there is at least one citation describing their use on paraffin tissue sections (Michelle L. Schlief, Ann Marie Craig, and Jonathan D. Gitlin. NMDA Receptor Activation Mediates Copper Homeostasis in Hippocampal Neurons. The Journal of Neuroscience, January 5, 2005, 25(1):239 - 246).
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
The dyes are proprietary, however they are stains that label the Nissl substance, which is composed of ribosomal RNA associated with the rough endoplasmic reticulum and is present in high amounts in neuronal cells.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Please check out this web page (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/neuronal-tracing.html) for details.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Citations & References (21)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
Transgenic labeling of the corticospinal tract for monitoring axonal responses to spinal cord injury.
Journal:Nat Med
PubMed ID:16286922
'The rodent corticospinal tract (CST) has been used extensively to investigate regeneration and remodeling of central axons after injury. CST axons are currently visualized after injection of tracer dye, which is invasive, incomplete and prone to variation, and often does not show functionally crucial but numerically minor tract components. Here,
A new method for imaging and 3D reconstruction of mammalian cochlea by fluorescent confocal microscopy.
Journal:Brain Res
PubMed ID:15053969
'Traditional methods for anatomical and morphometric studies of cochlear tissues have relied upon either microdissection of the organ of Corti or the generation of serial sections of the cochlea. Such methods are time-consuming, disruptive to three-dimensional relationships and often restrict sampling to very limited numbers of cells. We have found
Characterization of nestin expression in the spinal cord of GFP transgenic mice after peripheral nerve injury.
Journal:Neuroscience
PubMed ID:20673789
'Many studies have shown that activation and increase in the number of astrocytes and microglia in the spinal cord participate in the initiation and maintenance of neuropathic pain, but little attention has been paid to the responses of neural progenitor cells to peripheral nerve injury. Nestin, a class VI intermediate
Synfire chains and cortical songs: temporal modules of cortical activity.
Journal:Science
PubMed ID:15105494
How can neural activity propagate through cortical networks built with weak, stochastic synapses? We find precise repetitions of spontaneous patterns of synaptic inputs in neocortical neurons in vivo and in vitro. These patterns repeat after minutes, maintaining millisecond accuracy. Calcium imaging of slices reveals reactivation of sequences of cells during
RNAi reveals doublecortin is required for radial migration in rat neocortex.
Journal:Nat Neurosci
PubMed ID:14625554
Mutations in the doublecortin gene (DCX) in humans cause malformation of the cerebral neocortex. Paradoxically, genetic deletion of Dcx in mice does not cause neocortical malformation. We used electroporation of plasmids encoding short hairpin RNA to create interference (RNAi) of DCX protein in utero, and we show that DCX is
21 total citations