Pluronic F-127 is a nonionic, surfactant polyol (molecular weight approximately 12,500 daltons) that has been found to facilitate the solubilizationRead more
Pluronic F-127 is a nonionic, surfactant polyol (molecular weight approximately 12,500 daltons) that has been found to facilitate the solubilization of water-insoluble dyes and other materials in physiological media.1 Pluronic F-127 is commonly used to help disperse the acetoxymethyl (AM) esters of our ion indicators as well as our cell tracer dyes such as CFDA-SE.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Physical FormPowder
Recommended StorageStore at room temperature.
Quantity2 g
Unit SizeEach
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Lot #Certificate TypeDateCatalog Number(s)
3148393Certificate of AnalysisJun 18, 2025P6867
2984203Certificate of AnalysisAug 16, 2024P6867
2712262Certificate of AnalysisAug 31, 2023P6867
2519456Certificate of AnalysisSep 26, 2022P6867
2421167Certificate of AnalysisDec 21, 2021P6867
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Citations & References
Abstract
Rescue of vasopressin V2 receptor mutants by chemical chaperones: specificity and mechanism.
Authors:Robben JH,Sze M,Knoers NV,Deen PM
Journal:Molecular biology of the cell
PubMed ID:16267275
Because missense mutations in genetic diseases of membrane proteins often result in endoplasmic reticulum (ER) retention of functional proteins, drug-induced rescue of their cell surface expression and understanding the underlying mechanism are of clinical value. To study this, we tested chemical chaperones and sarco(endo)plasmic reticulum Ca(2+) ATPase pump inhibitors on ... More
Indo-1 binding to protein in permeabilized ventricular myocytes alters its spectral and Ca binding properties.
Authors:Hove-Madsen L, Bers DM
Journal:Biophys J
PubMed ID:1420876
We have examined the binding of the fluorescent Ca indicator indo-1 to cellular protein in permeabilized ventricular myocytes and also to soluble and particulate myocyte protein. Using either a filtration technique or equilibrium dialysis, and conditions similar to those in a cardiac myocyte patch clamped with 100 microM indo-1 in ... More
Long-term regulation of neuronal calcium currents by prolonged changes of membrane potential.
Authors:Franklin JL, Fickbohm DJ, Willard AL
Journal:J Neurosci
PubMed ID:1315850
'Although rapid-onset, short-term regulation of neuronal Ca currents by neurotransmitters and second messengers is well documented, little is known about conditions that can cause longer-lasting changes in Ca channel function. We report here that persistent depolarization is accompanied by slowly developing long-term reduction of neuronal Ca currents. Rat myenteric neurons ... More
Regulation of cytosolic free calcium concentration by intrasynaptic mitochondria.
Authors:Martínez-Serrano A, Satrústegui J
Journal:Mol Biol Cell
PubMed ID:1550964
'By the use of digitonin permeabilized presynaptic nerve terminals (synaptosomes), we have found that intrasynaptic mitochondria, when studied "in situ," i.e., surrounded by their cytosolic environment, are able to buffer calcium in a range of calcium concentrations close to those usually present in the cytosol of resting synaptosomes. Adenine nucleotides ... More
Intracellular astrocyte calcium waves in situ increase the frequency of spontaneous AMPA receptor currents in CA1 pyramidal neurons.
Authors:Fiacco TA, McCarthy KD
Journal:J Neurosci
PubMed ID:14736858
'Spontaneous neurotransmitter release and activation of group I metabotropic glutamate receptors (mGluRs) each play a role in the plasticity of neuronal synapses. Astrocytes may contribute to short- and long-term synaptic changes by signaling to neurons via these processes. Spontaneous whole-cell AMPA receptor (AMPAR) currents were recorded in CA1 pyramidal cells ... More