Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents
Use 96- and 384-well Microplates for Fluorescence-based Assays with Quant-iT assays for optimal results
For accurate quantification and a streamlined workflow try our new Quant-iT PicoGreen ReadyPlates!
Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents
Invitrogen™

Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents

Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent.
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Catalog NumberQuantityProduct TypePackaging Type
P7581Promo Image1 mLdsDNA Reagent1 x 1 mL Bottle
P7589Promo Image1 mL kitdsDNA Assay Kit1 x 1 mL Bottle
P11496Promo Image10 x 100 μL kitdsDNA Assay Kit10 x 100 μL Tubes
P11495Promo Image10 x 100 μLdsDNA Reagent10 x 100 μL Tubes
Catalog number P7581
Price (USD)
592.65
Online Exclusive
620.00
Save 27.35 (4%)
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Quantity:
1 mL
Product Type:
dsDNA Reagent
Packaging Type:
1 x 1 mL Bottle
Price (USD)
592.65
Online Exclusive
620.00
Save 27.35 (4%)
Each
Add to cart
Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Picogreen assays are compatible with most microplate readers and fluorometers.
Rapidly detect as little as 0.25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA, miniprep DNA, or PCR amplification products.

PicoGreen dsDNA quantitation reagents are
• Significantly more sensitive than UV absorbance readings, saving precious sample amounts
• Specific for dsDNA in the presence of RNA
• Easy to use—just add the dye working solution to the sample, incubate five minutes, and read
• Suitable for use in 96- and 384-well plate formats
• Compatible with most fluorescence-based microplate readers and fluorometers
• Picogreen aproximate fluorescence absorption/emission maxima is 502/523 nm, bound to dsDNA

Applications
The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA quantitation, measuring dsDNA in complex mixtures, and viral DNA quantitation.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Excitation/Emission500/525
For Use With (Equipment)Microplate Reader
No. of Reactions200 Assays (2 mL assay volume)
Packaging Type1 x 1 mL Bottle
Product LinePICOGREEN, Quant-iT
Product TypedsDNA Reagent
Quantitation Range50 pg to 2 μg
Quantity1 mL
Shipping ConditionRoom Temperature
Detection MethodFluorescence
Unit SizeEach

Frequently asked questions (FAQs)

Why am I getting negative fluorescence values with my Qubit Assays?

Negative fluorescence is a physical impossibility. It is an artifact from software autocorrecting for background signal. This means your reader is picking up and subtracting out background light at the cost of your data. Make sure to do a buffer-only control and assess the type of signal. You may need to switch to a different plate.

I have a Quant-iT DNA Kit and want to use it for the Qubit Fluorometer. Can I?

Yes, the manual has directions for this application. You will use the 0 ng/µL lambda dsDNA HS standard to generate Standard #1. You will prepare a dilution of the 10 ng/µL lambda dsDNA HS standard to generate Standard #2. You then prepare the samples and compare them to this 2-point standard curve. The Quant-iT dsDNA BR Kit can be used in a similar manner.

What is the useful pH range for Quant-iT DNA kits?

The buffer included in the kit should assure the proper pH range, even if your DNA is at a pH outside of this range, since at least a 10-fold excess of kit buffer over sample is used in the assay.

I'm trying to quantify some DNA labeled with a fluorophore. Will this work?

PicoGreen dye and other fluorescence-based quantification reagents are not recommended for quantifying dye-conjugated nucleic acids. The attached dye molecules can interfere with either binding and/or fluorescence output of the quantification reagents.

Does DNA length have an effect on the dsDNA assays?

Strands that are roughly in the 20-mer range or shorter show a lower level of signal. For dsDNA samples that are composed of mostly short strands, the reagent may still be used, but one should use a dsDNA standard that is of comparable length as the sample.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

Fluorescence spectra

Fluorescence spectra

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