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Application
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TSP50 Antibody (PA5-47316) in WB
Western blot analysis of TSP50 in human testis tissue. Samples were incubated in TSP50 polyclonal antibody (Product # PA5-47316) using a dilution of 0.2 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for TSP50 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions.
Product Details
PA5-47316
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TSP50 Ala40-Ala363
Conjugate
Unconjugated
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2576682
Product Specific Information
In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant mouse TSP50 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
Testes-specific protease 50 (TSP50) is a gene located within the chromosome region 3p21.31, primarily expressed in testicular tissue, and is homologous to serine proteases. However, TSP50 contains a distinctive catalytic triad where serine, a crucial catalytic residue, is replaced by threonine, allowing it to exhibit a novel protease activity. TSP50 is recognized for its role as a pro-oncogene, contributing significantly to cancer progression by promoting aerobic glycolysis, known as the Warburg effect, in hepatocellular carcinoma cells through interaction with pyruvate kinase M2 isoform (PKM2). This function underlines its involvement in cancer metabolism, as it attenuates colitis by regulating TGF-ß signaling pathways. Furthermore, studies have linked TSP50 to cancer stem cell maintenance, driving stem-like properties in breast cancer cells, thereby facilitating tumor initiation and self-renewal capabilities. These characteristics highlight TSP50's potential as a target in therapeutic interventions for cancer treatment.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cancer/testis antigen 20; Probable threonine protease PRSS50; protease, serine, 50; Serine protease 50; testes specific protease 50; testes-specific protease 50; testicular tissue protein Li 210; Testis-specific protease-like protein 50
Gene Aliases: CT20; PRSS50; TSP50
UniProt ID: (Human) Q9UI38
Entrez Gene ID: (Human) 29122
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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