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HB9 Antibody (PA5-67195) in ICC/IF
Immunofluorescence analysis of HB9 was performed using 70% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with HB9 Polyclonal Antibody (Product # PA5-67195) at 0.2 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1... View More
Product Details
PA5-67195
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human HLXB9. Recombinant protein control fragment (Product #RP-107833).
Conjugate
Form
Liquid
Concentration
0.10 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2662922
Product Specific Information
Immunogen sequence: NFRIDALLAVD PPRAASAQSA PLALVTSLAA AASGTGG
Highest antigen sequence identity to the following orthologs - mouse 84%, rat 87%.
Target Information
Homeobox protein HB9 is expressed in lymphoid and pancreatic tissues. It is reported to be Putative transcription factor involved in pancreas development and function. Defects in this gene causes Currarino syndrome. The triad of a presacral tumor, sacral agenesis and anorectal malformation constitutes the Currarino syndrome which is caused by dorsal-ventral patterning defects during embryonic development. It is also reported that HB9 is strongly expressed in epidermal basal cells of the outer scale face in tarsometatarsal scale skin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: homeobox HB9; Homeobox protein HB9; HOX SCRA1; Motor neuron and pancreas homeobox protein 1
Gene Aliases: HB9; HLXB9; HOXHB9; MNX1; SCRA1
UniProt ID: (Human) P50219
Entrez Gene ID: (Human) 3110
Performance Guarantee
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