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Invitrogen
HIF-1 beta Polyclonal Antibody
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 1
HIF-1 beta Antibody (PA5-81967)
Relative expression in different tissues in IHC: Detection of differential expression levels of HIF-1 beta demonstrates antibody specificity. Immunohistochemical analysis of HIF-1 beta using anti-HIF-1 beta Polyclonal Antibody (Product #PA5-81967), shows significant staining of HIF-1 beta in placenta and shows minimal or weak staining in pancreas tissues. The relative expression levels of HIF-1 beta within... View More
Product Details
PA5-81967
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human ARNT. Recombinant protein control fragment (Product #RP-100727).
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
Anitgen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2789128
Product Specific Information
Immunogen sequence: RFSCLRPRVA GTTEMTSDVP SLGPAIASGN SGPGIQGGGA IVQRAIKRRP GLDFDDDGEG NSKFLRCDDD QMSNDKERFA RSDDEQSSAD KERLARENHS EIERRRRNKM TAYITELSDM V
Target Information
HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. Diseases associated with HIF-1 beta dysfunction include hypoxia and renal cell carcinoma.HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. Diseases associated with HIF-1 beta dysfunction include hypoxia and renal cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ARNT protein; Aryl hydrocarbon receptor nuclear translocator; bHLHe2; Class E basic helix-loop-helix protein 2; Dioxin receptor, nuclear translocator; hif 1; HIF-1-beta; HIF1 beta; hypoxia-inducible factor 1, beta subunit; Hypoxia-inducible factor 1-beta
Gene Aliases: ARNT; BHLHE2; HIF-1-beta; HIF-1beta; HIF1-beta; HIF1B; HIF1BETA; TANGO
UniProt ID: (Human) P27540
Entrez Gene ID: (Human) 405
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
transcription coactivator activity
aryl hydrocarbon receptor activity
protein binding
transcription factor binding
aryl hydrocarbon receptor binding
enhancer binding
sequence-specific DNA binding
protein heterodimerization activity
basic helix-loop-helix transcription factor
response to hypoxia
embryonic placenta development
positive regulation of endothelial cell proliferation
transcription, DNA-templated
positive regulation of vascular endothelial growth factor production
cell differentiation
intracellular receptor signaling pathway
positive regulation of vascular endothelial growth factor receptor signaling pathway
positive regulation of protein sumoylation
mRNA transcription from RNA polymerase II promoter
regulation of transcription from RNA polymerase II promoter in response to oxidative stress
positive regulation of erythrocyte differentiation
positive regulation of glycolytic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of hormone biosynthetic process
regulation of transcription from RNA polymerase II promoter in response to hypoxia
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