Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
RASGRP2 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 2
RASGRP2 Antibody (PA5-82534) in WB
Western blot was performed using RASGRP2 Polyclonal Antibody (Product # PA5-82534) and a 69 kDa band corresponding to RAS Guanyl Releasing Protein 2 was observed in tested cell lines and tissues except SK-N-AS and SK-OV-3. Whole cell extracts (30 µg lysate) of KG-1 (Lane 1), Reh (Lane 2), SK-N-AS (Lane 3), SK-O-V3 (Lane 4) and Mouse Spleen (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a PVDF membrane (Product # IB24001) by iBlot® 2 Dry Blotting Sys... View More
Product Details
PA5-82534
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human RASGRP2. Recombinant protein control fragment (Product #RP-90658).
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
Anitgen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2789692
Product Specific Information
Immunogen sequence: DDSGKVRDPQ LVRMFLMMHP WYIPSSQLAA KLLHIYQQSR KDNSNSLQVK TCHLVRYWIS AFPAEFDLNP ELAEQIKELK ALLDQEGNRR HSSLIDIDSV PTYKWKRQVT QRNPV
Target Information
The protein encoded by this gene is a brain-enriched nucleotide exchanged factor that contains an N-terminal GEF domain, 2 tandem repeats of EF-hand calcium-binding motifs, and a C-terminal diacylglycerol/phorbol ester-binding domain. This protein can activate small GTPases, including RAS and RAP1/RAS3. The nucleotide exchange activity of this protein can be stimulated by calcium and diacylglycerol. Three alternatively spliced transcript variants encoding the same protein have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Calcium and DAG-regulated guanine nucleotide exchange factor I; calcium and diacylglycerol-regulated guanine nucleotide exchange factor I; CalDAG-GEFI; Cdc25-like protein; F25B3.3 kinase like protein; F25B3.3 kinase-like protein; guanine exchange factor MCG7; hCDC25L; RAP 1A protein-specific guanine nucleotide exchange factor 1; RAS guanyl nucleotide-releasing protein 2; RAS guanyl releasing protein 2 (calcium and DAG-regulated); RAS guanyl-releasing protein 2; RAS, guanyl releasing protein 2; RAP 1A protein-specific guanine nucleotide exchange factor 1; CalDAG-GEFI
Gene Aliases: CALDAG-GEFI; Caldaggef1; CDC25L; MCG7; RASGRP2
UniProt ID: (Human) Q7LDG7, (Mouse) Q9QUG9
Entrez Gene ID: (Human) 10235, (Mouse) 19395
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support