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Application
FIGURE: 1 / 1
DZIP3 Antibody (PA5-96601) in WB
Western blot analysis of DZIP3 in extracts of 293T cells. Samples were incubated with DZIP3 Polyclonal antibody (Product # PA5-96601) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Product Details
PA5-96601
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human DZIP3 (NP_0554631)
Conjugate
Unconjugated
Form
Liquid
Concentration
0.64 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2808403
Product Specific Information
Immunogen sequence: MDSLPDEFFV RHPAVEDQRK EETENKLEKS SGQLNKQEND IPTDLVPVNL LLEVKKLLNA INTLPKGVVP HIKKFLQEDF SFQTMQREVA ANSQNGEEIV PALTLRFLIT QLEAALRNIQ AGNYTAHQIN IGYYLTLLFL YGVALTERGK KEDYTEAENK FLVMKMMIQE NEICENFMSL VYFGRGLLRC AQKRYNGGLL EFHKSLQEIG DKNDHWFDID PTEDEDLPTT; Positive Samples: 293T; Cellular Location: Cytoplasm
Target Information
E3 Ubiquitin ligase proteins mediate ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Able to specifically bind RNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DAZ interacting protein 3, zinc finger; DAZ-interacting protein 3; E3 ubiquitin-protein ligase DZIP3; hRUL138; human RNA-binding ubiquitin ligase of 138 kDa; protein phosphatase 1, regulatory subunit 66; RING-type E3 ubiquitin transferase DZIP3; RNA-binding RING-H2 protein-ubiquitin ligase; RNA-binding ubiquitin ligase of 138 kDa; UURF2 ubiquitin ligase; zinc finger DAZ interacting protein 3
Gene Aliases: DZIP3; hRUL138; KIAA0675; PPP1R66; UURF2
UniProt ID: (Human) Q86Y13
Entrez Gene ID: (Human) 9666
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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