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Product Details
PHC0011
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
SAPFSFLSNV KYNFMRIIKY EFILNDALNQ SIIRANDQYL TAAALHNLDE AVKFDMGAYK SSKDDAKITV ILRISKTQLY VTAQDEDQPV LLKEMPEIPK TITGSETNLL FFWETHGTKN YFTSVAHPNL FIATKQDYWV CLAGGPPSIT DFQILENQA
Molecular weight
18 kDa
Class
Recombinant
Type
Protein
Purity
>95% by SDS-PAGE
Endotoxin concentration
<0.1 ng/µg
Activity
ED50 = 0.001 - 0.005 ng/mL; determined by the dose-dependent proliferation of mouse D10S cells.
Conjugate
Form
Lyophilized
Purification
purified
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Wet ice (domestic); Wet or Dry ice (international)
Product Specific Information
Carrier-Free
Reconstitution: We recommend that the vial be briefly centrifuged prior to opening to bring the contents to the bottom. Lyophilized human IL-1 alpha should be reconstituted in deionized water to 0.1-1.0 mg/mL to regain full activity. These stock solutions should be apportioned into working aliquots and stored at ≤-20°C. Further dilutions should be made in low endotoxin medium or buffered solution with heat inactivated FCS or tissue culture grade BSA. Storage: Lyophilized human IL-1 alpha should be stored at 2°C to 8°C, preferably desiccated. Store reconstituted human IL-1 alpha at ≤-20°C (not in a frost-free freezer). Keep freeze-thaw cycles to a minimum.
Target Information
IL-1 alpha (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 alpha is coded by the IL1A gene and signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 beta. IL1A is located on the q arm on chromosome 2 at position 13. IL-1 alpha plays an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. IL-1 alpha activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. Further, IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury and induces apoptosis. The IL1A gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. IL1 alpha regulates the activities of NF-kappaB and mitogen-activated protein kinases. IL-1 alpha can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-1 alpha are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CTLA8; Hematopoietin-1; IL 1 a; IL 1α; IL-1 alpha; IL17; IL1α; ILN; Interleukin; interleukin 1, alpha; interleukin 17; Interleukin-1 alpha; Interleukin1 alpha; preinterleukin 1 alpha; pro-interleukin-1-alpha; RP23-160G19.8
Gene Aliases: IL-1A; IL1; IL1-ALPHA; IL1A; IL1F1
UniProt ID: (Human) P01583
Entrez Gene ID: (Human) 3552
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