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Product Details
PV6243
Product Specifications
Species
Human
Expression System
Baculovirus
Amino acid sequence
10-end
Tag
His-tag
Molecular weight
63.6-38-51 kDa
Class
Recombinant
Type
Protein
Conjugate
Form
Liquid
Concentration
See Label
Purification
purified
Storage buffer
50mM sodium phosphate, pH 7, with 150mM Imidazole, 25% glycerol, 300mM NaCl, 0.1mM PMSF, 0.2mM DTT
Contains
no preservative
Storage conditions
-80° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Dry ice
Product Specific Information
For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.
Recombinant full-length human AMPK (combination of A1/B1/G3 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tag.
• Validated in LanthaScreen™ Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use
Related Product:
LanthaScreen™ Eu Kinase Binding Assay
Protein Form: Recombinant
Target Information
AMPK (A2/B2/G3) is a member of the AMPK family which are heterotrimeric proteins consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPKs are an important energy-sensing enzyme group in the cells that monitor energy status particularly in response to stress. AMPKs regulate fatty acid and cholesterol synthesis by regulating the key rate-limiting enzymes acetyl-CoA carboxylase and hydroxy beta-methylglutaryl-CoA reductase. The beta subunit may be a positive regulator of AMPK activity and is highly expressed in skeletal muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 5'-AMP-activated protein kinase beta-1 subunit; 5'-AMP-activated protein kinase catalytic subunit alpha-1; 5'-AMP-activated protein kinase subunit beta-1; 5'-AMP-activated protein kinase subunit gamma-3; 5'-AMP-activated protein kinase, catalytic alpha-1 chain; 5'-AMP-activated protein kinase, gamma-3 subunit; ACACA kinase; Acetyl-CoA carboxylase kinase; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase beta subunit; AMP-activated protein kinase, catalytic, alpha-1; AMPK alpha 1; AMPK alpha 1/beta 1/gamma 3; AMPK alpha1/beta1/gamma3; AMPK beta -1 chain; AMPK beta 1; AMPK gamma-3 chain; AMPK gamma3; AMPK subunit alpha-1; AMPK subunit beta-1; AMPK subunit gamma-3; AMPKb; HMGCR kinase; Hydroxymethylglutaryl-CoA reductase kinase; protein kinase, AMP-activated, alpha 1 catalytic subunit; protein kinase, AMP-activated, beta 1 non-catalytic subunit; protein kinase, AMP-activated, gamma 3 non-catalytic subunit; protein kinase, AMP-activated, noncatalytic, beta-1; Tau-protein kinase PRKAA1
Gene Aliases: AMPK; AMPK1; AMPKa1; AMPKG3; HAMPKb; PRKAA1; PRKAB1; PRKAG3
UniProt ID: (Human) Q13131, (Human) Q9Y478, (Human) Q9UGI9
Entrez Gene ID: (Human) 5562, (Human) 5564, (Human) 53632
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