Yeast Nitrogen Base

Yeast nitrogen base (YNB) is a base medium for preparation of minimal and synthetic defined yeast media. YNB contains ammoniumRead more

Catalog Number	Quantity
Q30007	1 Pouch

Catalog number Q30007

Price (USD)

268.00

Each

Add to cart

Quantity:

1 Pouch

Price (USD)

268.00

Each

Add to cart

Yeast nitrogen base (YNB) is a base medium for preparation of minimal and synthetic defined yeast media. YNB contains ammonium sulfate but does not contain amino acids. Both bulk and convenient single-use pouches are available.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Quantity1 Pouch

FormPowder

Product TypeMicrobiology Media

Target Organism ClassP. pastoris, S. cerevisiae

Unit SizeEach

Contents & Storage

Each pouch contains 67 g of reagent to prepare 500 mL of 10X YNB for Pichia media or 1,000 mL of 10X YNB for S. cerevisiae media.

Store at room temperature. Guaranteed stable for 6 months when properly stored.

This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our Privacy Notice.

Frequently asked questions (FAQs)

What are the components and concentrations for 1X YNB (yeast nitrogen base)?

For the pouches sold as Cat. No. Q30007, each pouch contains reagents to prepare 500 mL of 10X YNB for Pichia media or 1,000 mL of 10X YNB for S. cerevisiae media (since Pichia media is 2X what is used for S. cerevisiae). Below is the formulation for 1X YNB:

Ammonium sulfate, 5 g
Biotin, 0.002 mg
Calcium pantothenate, 0.4 mg
Folic acid, 0.002 mg
Inositol, 2 mg
Niacin, 0.4 mg
Para-aminobenzoic acid, 0.2 mg
Pyridoxine HCl, 0.4 mg
Riboflavin, 0.2 mg
Thiamine HCl, 0.4 mg
Boric acid, 0.5 mg
Copper sulfate, 0.4 mg
Potassium iodide, 0.1 mg
Ferric chloride.6H2O, 0.2 mg
Manganese sulfate.H2O, 0.4 mg
Sodium molybdate.2H2O, 0.2 mg
Zinc sulfate.H2O, 0.4 mg
Potassium phosphate monobasic, 1 g
Magnesium sulfate, 0.5 g
Sodium chloride, 0.1 g
Calcium chloride, 0.1 mg

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Can YNB medium (with ammonium sulfate, without amino acids) be autoclaved instead of filter-sterilized?

Yes, YNB medium with ammonium sulfate and without amino acids can be autoclaved for sterilization.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Yeast Nitrogen Base

Frequently asked questions (FAQs)

What are the components and concentrations for 1X YNB (yeast nitrogen base)?

Can YNB medium (with ammonium sulfate, without amino acids) be autoclaved instead of filter-sterilized?

Documents & Downloads

Certificates

Safety Data Sheets